Abstract
Cationic surfactants precipitate nucleic acids, presumably by forming reverse micelles in which the ahphatic tails of the surfactant face the aqueous phase, and the cationic head groups bind to the nucleic acid electrostatically. The precipitate can be dissolved in organic solvents, or it can be redissolved in water by the addition of salt. Selected cationic surfactants are capable of lysing cells, by solubilizmg their protem and lipid components.
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References
Macfarlane D. E. and Dahle C. E. (1993) Isolating RNA from whole blood—the dawn of RNA-based diagnosis? Nature 362, 186–188.
Dahle C. E. and Macfarlane D. E. (1993) Isolation of RNA from cells in culture using Catrimox-14 catiomc surfactant. BioTechniques 15, 1102–1105.
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© 1998 Humana Press Inc.
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Dahle, C.E., Macfarlane, D.E. (1998). Isolating RNA with the Cationic Surfactant, Catrimox-14. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:19
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DOI: https://doi.org/10.1385/0-89603-494-1:19
Publisher Name: Humana Press
Print ISBN: 978-0-89603-494-5
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