Abstract
Nonradioactively labeled probes offer several advantages compared to radioactive ones, as they show long stability, high morphologrcal resolution, and rapid developing time. There are different types of nonradioactive labeling methods available, although dlgoxigenin-labeled probes (1) have become the most widely used for investigation on animal tissue, as they offer the advantage of low background noise and increased sensitivity (2,3). Also, digoxigenin can be used to label either RNA, DNA, or oligonucleotide probes. There have been different opinions on the senstilvity of detection of dlgoxrgenin probes, but it has been shown that the sensitivity of radiolabeled and nonradioactive probes is comparable (3).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kessler C (1991) The digoxtgenin anti-digoxigenin (DIG) technology—A survey on the concept and realization of a novel bioanalytical indicator system. Mol Cell Probes 5, 16l–205
Morris R G, Arends M. J., Bishop P. E., Sizer K., Duvall E, and Bird C. C. (1990) Sensitivity of digoxigenin and biotin labeled probes for detection of human paptllomavirus by in situ hybridization. J. Clin. Pathol 43, 800–805.
Furuta Y., Shinohara T., Sano K., Meguro M., and Nagashima K. (1990) in situ hybridization with digoxigenin-labeled DNA probes for detection of viral genomes. J Clin. Pathol. 43, 806–809
Giaid A., Hamid Q., Adams C., Springall D. R., Terenghi G., and Polak J. M (1989) Non-isotopic RNA probes. Comparison between different labels and detection systems. Histochemistry 93, 191–196
Dirks R. W. van Gijlswijk R. P. M. Tullis R. H. Smit A. B. van Minnen J. van der Ploeg M. and Raap A. K 1990 Simultaneous detection of different mRNA sequences coding for neuropeptide hormones by double in situ hybridization using FITC-and biotin-labeled oligonucleotides J Histochem Cytochem 38 467–47
Herrmgton C. S., Bums J., Graham A. K., Bhatt B., and McGee J. O. D. (1989) Interphase cytogenetlcs using biotin and digoxlgenin labeled probes. II. Simultaneous detection of two nucleic acid species in individual nucleic. J. Clin Pathol. 42, 601–606.
Trask B. J., Massa H., Kenwrick S., and Gitschier J. (1991) Mapping of human chromosome Xq28 by two colour fluorescence in situ hybridization of DNA sequences to interphase cell nucler. Am. J. Hum. Genet. 48, 1–15.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc.
About this protocol
Cite this protocol
Terenghi, G. (1998). Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:137
Download citation
DOI: https://doi.org/10.1385/0-89603-494-1:137
Publisher Name: Humana Press
Print ISBN: 978-0-89603-494-5
Online ISBN: 978-1-59259-570-9
eBook Packages: Springer Protocols