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The Xenopus Egg Extract Translation System

  • Glenn M. Matthews
  • Alan Colman
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 86)

Abstract

A full analysis of the post-translational modifications that a given protein undergoes during transit through the secretory pathway may, in some cases, only be performed by analysis of the natural protein, expressed in its normal tissue.

Keywords

Creatine Phosphate Translation Product Translation Reaction Sucrose Gradient Fractionation Signal Sequence Cleavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Matthews G. M. and Colman A (1991) A highly efficient, cell-free translation/translocation system prepared fromXenopus eggs. Nucleic Acids Res. 19, 6405–6412.PubMedCrossRefGoogle Scholar
  2. 2.
    Murray A. W. (1991) Cell cycle extracts, in Methods in Cell Biology, vol. 36 (Kay B. K. and Peng H. B., eds.), Academic, San Diego, pp. 581–605.Google Scholar
  3. 3.
    Kreig P. A. and Melton D. A. (1984) Functional messenger RNAs are produced by SP6 in vitro transcnptlon of cloned cDNAs Nucleic Acids Res 12, 7057–7070.CrossRefGoogle Scholar
  4. 4.
    Colman A. (1984) Translation of eukaryotic messenger RNA in Xenopus oocytes, in Transcription and Translation-A Practxal Approach (Hames B. D. and Higgins S. J., eds.), IRL, Oxford, pp. 271–302.Google Scholar

Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Glenn M. Matthews
  • Alan Colman
    • 1
  1. 1.School of BiochemistryBirmingham UniversityBirminghamUK

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