Abstract
With the discovery of the role of polyphosphoinositide hydrolysis in mediating the action of calcium-mobilizing hormones, growth factors, and neurotransmitters (reviewed in ref. 1), the development of methods for measuring the formation of the resultant second messengers, inositol 1,4,5-trisphosphate and diacylglycerol (DAG), became essential. The additional discovery of an effector enzyme, protein kinase C, for transducing the changes in DAG levels (reviewed in ref. 2) underlined the importance of this second messenger. A sensitive and reliable protocol currently in use in many laboratories is described below for the measurement of DAG levels in stimulated cells.
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© 1998 Humana Press Inc., Totowa, NJ
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Bollag, W.B., Griner, R.D. (1998). Measurement of Cellular Diacylglycerol Content. In: Bird, I.M. (eds) Phospholipid Signaling Protocols. Methods in Molecular Biology™, vol 105. Humana Press. https://doi.org/10.1385/0-89603-491-7:89
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DOI: https://doi.org/10.1385/0-89603-491-7:89
Publisher Name: Humana Press
Print ISBN: 978-0-89603-491-4
Online ISBN: 978-1-59259-255-5
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