Abstract
In Chapters 1 and 2, the extraction procedures for recovery of the phosphoinositols and phosphoinositides from cells in suspension or culture are described, together with simple separation procedures to resolve them into their general classes (InsP1, InsP2, and so on, and PtdIns, PtdInsP, and PtdInsP2). However, in reality, the metabolism of the phosphoinositols is complex (1) leading to the formation of several isomeric forms in each class. Furthermore, since the discovery of a phosphoinositide 3-kinase (in addition to the previously known 4-and 5-kinases) (2), it is clear that the phosphoinositides also exist in different isomeric forms.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Shears, S. B. (1989) Metabolism of the inositol phosphates produced upon receptor activation. Biochem. J. 260, 313–324.
Whitman, M., Downes, C. P., Keeler, M., Keeler, T., and Cantley, L. (1987). Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol 3-phosphate. Nature 332, 644–646.
Brown, J. E., Rudnick, M., Letcher, A. J., and Irvine, R. F. (1988) Formation of methylphosphoryl inositol phosphates by extractions that employ methanol. Biochem. J. 253, 703–710.
Stephens, L. R., Hawkins, P. T., and Downes, C. P. (1989) Metabolic and structural evidence for the existence of a third species of polyphosphoinositidein cells: D-phosphatidyl-myo-inositol 3-phosphate. Biochem. J. 259, 267–276.
Bird, I. M., Williams, B. C., and Walker, W. W. (1992) Identification and metabolism of phosphoinositol species formed on angiotensin II stimulation of zona fasciculata-reticularis cells from the bovine adrenal cortex. Mol. Cell. Endocrinol. 83, 29–38.
Bird, I. M., Nicol, M., Williams, B. C., and Walker, S. W. (1990) Vasopressin stimulates cortisol secretion and phosphoinositide catabolism in cultured bovine adrenal fasciculata/reticularis cells. J. Mol. Endocrinol. 5, 109–116.
Batty, I. A., Letcher, A. J., and Nahorski, S. R. (1989) Accumulation of inositol polyphosphate isomers in agonist-stimulated cerebral cortex slices. Biochem. J. 258, 23–32.
Dean, N. M. and Moyer, J. D. (1987) Separation of multiple isomers of inositol phosphates formed in GH3 cells. Biochem. J. 242, 361–366.
Irvine, R. F., Anggard, E. E., Letcher, A. J., and Downes, C. P. (1985) Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands. Biochem. J. 229, 505–511.
Batty, I. R., Nahorski, S. R., and Irvine, R. F. (1985) Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortex slices. Biochem. J. 232, 211–215.
Irvine, R. F., Letcher, A. J., Heslop, J. P., and Berridge, M. J. (1986) The inositol tris/tetrakisphosphate pathway—demonstration of Ins(1,4,5)P3 3-kinase activity in animal tissues. Nature 320, 631–634.
Hawkins, P. T., Stephens, L., and Downes, C. P. (1986) Rapid formation of inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands may both result indirectly from receptor-stimulated release of inositol 1,4,5-trisphosphate from phosphatidylinositol 4,5-bisphosphate. Biochem. J. 238, 507–516.
Bird, I. M., Smith, A. D., and Schulster, D. (1987) H.P.L.C. analysis of inositol monophosphate isomers formed on angiotensin II stimulation of rat adrenal glomerulosa cells. Biochem. J. 248, 203–208.
Siess, W. (1985) Evidence for the formation of inositol 4-monophosphate in stimulated human platelets. FEBS Letts. 185, 151–156.
Shears, S. B., Kirk, C. J., and Michell, R. H. (1987) The pathway of myo-inositol 1,3,4-trisphosphate dephosphorylation in liver. Biochem. J. 248, 977–980.
Stephens, L. R., Hawkins, P. T., and Downes, C. P. (1989b) An analysis of myo-[3H]inositol trisphosphates found in myo-[3H]inositol prelabeled avian erythrocytes. Biochem. J. 262, 727–737.
Stephens, L. R., Hawkins, P. T., Barker, C. J., and Downes, C. P. (1988) Synthesis of myo-inositol 1,3,4,5,6-pentakisphosphate from inositol phosphates generated by receptor activation. Biochem. J. 253, 721–733.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Bird, I.M. (1998). Phosphoinositidase C Activation Assay III. In: Bird, I.M. (eds) Phospholipid Signaling Protocols. Methods in Molecular Biology™, vol 105. Humana Press. https://doi.org/10.1385/0-89603-491-7:25
Download citation
DOI: https://doi.org/10.1385/0-89603-491-7:25
Publisher Name: Humana Press
Print ISBN: 978-0-89603-491-4
Online ISBN: 978-1-59259-255-5
eBook Packages: Springer Protocols