Skip to main content

Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis

  • Protocol
Phospholipid Signaling Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 105))

Abstract

Sphingomyelin (SM) is the latest addition to the family of membrane phospholipids that is a source of molecules behaving as intracellular messengers (1). Ceramide is one of the products of sphingomyelin hydrolysis by specific sphingomyelinases (SMases) and appears to be involved in the signaling of apoptosis initiated by regulatory molecules that include cytokines, interferon-γ (IFN-γ), and chemotherapy drugs, as well as UV and ionizing radiations (24).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Spiegel, S., Foster, D., and Kolesnick R. (1996) Signal transduction through lipid second messengers. Curr. Opin. Cell Biol. 8, 159–167.

    Article  PubMed  CAS  Google Scholar 

  2. Haimovitz-Friedman, A., Kan, C-C., Ehleiter, D., Persaud, R. S., McLoughlin M., Fuks, Z., and Kolesnick, R. N. (1994) Ionizing radiation acts on cellular membranes to generate ceramide and initiate apoptosis. J. Exp. Med. 180, 525–535.

    Article  PubMed  CAS  Google Scholar 

  3. Bose, R., Verheij, M., Haimovitz-Friedman, A., Scotto, K., Fucks, Z., and Kolesnick, R. (1995) Ceramide synthase mediates daunorubicin-induced apoptosis: an alternative mechanism for generating death signals. Cell 82, 405–414.

    Article  PubMed  CAS  Google Scholar 

  4. Santana, P., Peña, L. A., Haimovitz-Friedman, A., Martin, S., Green, D., McLoughlin, M., Cordon-Cardo, C., Schuman E. H., Fucks, Z., and Kolesnick, R. (1996) Acid sphingomyelinase-deficient human lymphoblasts and mice are defective in radiation-induced apoptosis. Cell 86, 189–199.

    Article  PubMed  CAS  Google Scholar 

  5. Bligh, E. G. and Dyer, W. J. (1959) A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  6. Yavin, E. (1976) Regulation of phospholipid metabolism in differentiating cells from rat brain cerebral hemispheres in culture. Patterns of acetylcholine, phosphocholine and phosphoglycerides labeling from [methyl-14C]choline. J. Biol. Chem. 251, 1392–1397.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Santana, P., Fanjul, L.F., de Ruiz Galarreta, C.M. (1998). Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis. In: Bird, I.M. (eds) Phospholipid Signaling Protocols. Methods in Molecular Biology™, vol 105. Humana Press. https://doi.org/10.1385/0-89603-491-7:217

Download citation

  • DOI: https://doi.org/10.1385/0-89603-491-7:217

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-491-4

  • Online ISBN: 978-1-59259-255-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics