Abstract
We have summarized in Chapter 16 the recent advances in the mechanisms of regulation of leukotriene and prostaglandin biosynthesis and their pathways of synthesis. This chapter focuses on the measurement of the leukotrienes and other LOX products by high-pressure liquid chromatography (HPLC). At this point, we also present a complete summary in Table 1 comparing the various approaches used for the measurements of arachidonic acid metabolites derived from the prostaglandin endoperoxide synthase and the lipoxygenase pathways, as presented in Chapters 16–18.
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References
Surette, M. E., Odeimat, A., Palmantier, R., Marleau, S., Poubelle, P. E., and Borgeat, P. (1994) Reverse phase HPLC analysis of arachidonic acid metabolites in plasma after stimulation of whole blood ex vivo. Anal. Biochem. 216, 392–400.
Borgeat, P., Picard, S., Vallerand, P., Bourgoin, S., Odeimat, A., Sirois, P., and Poubelle, P. E. (1990) Automated on-line extraction and profiling of lipoxygenase products of arachidonic acid by high performance liquid chromatography, in Methods in Enzymology. Arachidonate related lipid mediators, vol. 187 (Murphy, R. C. and Fitzpatrick, F., eds.), Academic, New York, pp. 98–116.
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© 1998 Humana Press Inc., Totowa, NJ
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Borgeat, P., Picard, S., Battistini, B., Sirois, P. (1998). Measurements of Arachidonic Acid Metabolites Derived from the Lipoxygenase Pathways by High-Pressure Liquid Chromatography. In: Bird, I.M. (eds) Phospholipid Signaling Protocols. Methods in Molecular Biology™, vol 105. Humana Press. https://doi.org/10.1385/0-89603-491-7:209
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DOI: https://doi.org/10.1385/0-89603-491-7:209
Publisher Name: Humana Press
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