Abstract
Arachidonic acid has been implicated as a second messenger in numerous systems, and many agonists stimulate the intracellular accumulation of arachidonic acid (1,2). Arachidonic acid may be generated by phospholipase A2 hydrolysis of phospholipids, or by hydrolysis of diacylglycerol through the sequential action of diacylglycerol lipase and monoacylglycerol lipase (3–6). These activities can be easily measured using sensitive radiometric assays as described herein.
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© 1998 Humana Press Inc., Totowa, NJ
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Wolf, B.A. (1998). Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases. In: Bird, I.M. (eds) Phospholipid Signaling Protocols. Methods in Molecular Biology™, vol 105. Humana Press. https://doi.org/10.1385/0-89603-491-7:167
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DOI: https://doi.org/10.1385/0-89603-491-7:167
Publisher Name: Humana Press
Print ISBN: 978-0-89603-491-4
Online ISBN: 978-1-59259-255-5
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