Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases

  • Bryan A. Wolf
Part of the Methods in Molecular Biology™ book series (MIMB, volume 105)


Arachidonic acid has been implicated as a second messenger in numerous systems, and many agonists stimulate the intracellular accumulation of arachidonic acid (1,2). Arachidonic acid may be generated by phospholipase A2 hydrolysis of phospholipids, or by hydrolysis of diacylglycerol through the sequential action of diacylglycerol lipase and monoacylglycerol lipase (3, 4, 5, 6). These activities can be easily measured using sensitive radiometric assays as described herein.


Arachidonic Acid Shake Water Bath Fume Hood Ferric Chloride Solution Rhizopus Arrhizus 
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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Bryan A. Wolf
    • 1
  1. 1.Department of Pathology and Laboratory MedicineUniversity of Pennsylvania School of MedicinePhiladelphia

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