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Isolation of Cytokine-Inducible Genes from Hematopoietic Cells by Differential Display

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Differential Display Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 85))

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Abstract

Hematopoietic stem cells have the capacity to divide and differentiate into several different hematopoietic cell lineages. Hematopoietic growth factors (cytokines) promote both the growth and differentiation of stem cells and committed progenitor cells. Cytokines bind and activate discrete receptors expressed on the surface of stem cells and thereby influence cell fate decisions.

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References

  1. Cosman, D., Lyman, S. D., Idzerda, R. L., and Beckmann, M. P. (1990) A New cytokine receptor superfamily. Trends Biochem. Sci. 15, 265–270.

    Article  PubMed  CAS  Google Scholar 

  2. Sato, N., Sakamaki, K., Terada, N., Arai, K., and Miyajima, A. (1993) signal transduction by the high affinity GM-CSF receptor: two distinct cytoplasmic regions of the common P subunit responsible for differentiation. EMBO J. 12, 4181–4189.

    PubMed  CAS  Google Scholar 

  3. Darnell, J. E., Kerr, I. M., and Stark, G. R. (1994) Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signalling proteins. Science 264, 1415–1421.

    Article  PubMed  CAS  Google Scholar 

  4. Ihle, J., Witthuhn, B. A., Quelle, F. W., Yamamoto, K., Thierfelder, W. E. Kreider, B., and Silvennoinen, O. (1994) Signalling by the cytokine receptor superfamily: JAKs and STATs. Trends Biochem. Sci. 19, 222–227.

    Article  PubMed  CAS  Google Scholar 

  5. Palacios, R. and Steinmetz, M. (1985) IL-3 dependent mouseclones that express B220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes. Cell 41, 727–734.

    Article  PubMed  CAS  Google Scholar 

  6. D’Andrea, A. D., Lodish, H. F., and Wong, G. G. (1989) Expression cloning of the murine erythropoietin receptor. Cell 57, 277.

    Article  PubMed  Google Scholar 

  7. Carroll, M., Zhu, Y., and D’Andrea, A. D. (1995) Erythropoietin-induced cellular differentiation requires prolongation of the Gl phase of the cell cycle. Proc. Natl. Acad. Sci. USA 92, 2869–2873.

    Article  PubMed  CAS  Google Scholar 

  8. Liboi, E., Carroll, M., D’Andrea, A. D., and Mathey-Prevot, B. (1993) The erythropoietin receptor signals both proliferation and erythroid-specific differentiation. Proc. Natl. Acad. Sci. USA 90, 11,351–11,355.

    Article  PubMed  CAS  Google Scholar 

  9. Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic mRNA by means of the polymerase chain reaction. Science 257, 967–971.

    Article  PubMed  CAS  Google Scholar 

  10. Liang, P., Averboukh, L., and Pardee, A. B. (1993) Distribution and cloning of eukaryotic mRNAs by means of differential diaplay: refinements and optimization. Nucleic Acids Res. 21, 3269–3275.

    Article  PubMed  CAS  Google Scholar 

  11. Zhu, Y., Carroll, M., Papa, F. R., Hochstrasser, M., and D’Andrea, A. D. (1996) DUB, a novel deubiquitinating enzyme with growth-suppressing activity. PNAS 93, 3275–3279.

    Article  PubMed  CAS  Google Scholar 

  12. Zhu, Y., Pless, M., Inhorn, R., Mathey-Prevot, B., and D’Andrea, A. D. The murine DUB-1 gene is specifically induced by the βc subunit of the interleukin-3 (IL-3) receptor. Mol. Cell. Biol., in press.

    Google Scholar 

  13. Kohno, H., Furukawa, T., Yoshinaga, T., Tokunaga, R., and Taketani, S. (1993) Coproporphyrinogen oxidase: purification, molecular cloning, and induction of mRNA during erythroid differentiation. J. Biol. Chem. 268, 21,359–21,363.

    PubMed  CAS  Google Scholar 

  14. Hayzer, D. J., Brinson, E., and Runge, M. S. (1992) A rat β-interteron-induced mRNA: sequence characterization. Gene 111, 271–278.

    Google Scholar 

  15. Lewin, A. R., Reid, L. E., McMahon, M., Stark, G. R., and Kerr, I. M. (1991) Molecular analysis of a human interferon-inducible gene family. Eur. J. Biochem. 199, 417–423.

    Article  PubMed  CAS  Google Scholar 

  16. McNeil, H. P., Austen, K. F., Somerville, L. L., Gurish, M. F., and Stevens, R. L. (1991) Molecular cloning of the mouse mast cell protease-5 gene. J. Biol. Chem. 266, 20,316–20,322.

    PubMed  CAS  Google Scholar 

  17. Priefert, H. and Steinbuchel, A. (1992) Identification and molecular characterization of the acetyl coenzyme A synthetase gene (acoE) of Alcaligenes eutrophus. J. Bacteriol. 174, 6590–6599.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Division of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA

    Yuan Zhu, Tom Hahn & Alan D. D’Andrea

Authors
  1. Yuan Zhu
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  2. Tom Hahn
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  3. Alan D. D’Andrea
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Editor information

Editors and Affiliations

  1. Vanderbilt Cancer Center, Nashville, TN

    Peng Liang

  2. Dana-Farber Cancer Institute, Boston, MA

    Arthur B. Pardee

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© 1997 Humana Press Inc.

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Zhu, Y., Hahn, T., D’Andrea, A.D. (1997). Isolation of Cytokine-Inducible Genes from Hematopoietic Cells by Differential Display. In: Liang, P., Pardee, A.B. (eds) Differential Display Methods and Protocols. Methods in Molecular Biology, vol 85. Humana Press. https://doi.org/10.1385/0-89603-489-5:153

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  • DOI: https://doi.org/10.1385/0-89603-489-5:153

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-489-1

  • Online ISBN: 978-1-59259-569-3

  • eBook Packages: Springer Protocols

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