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RC4D—Restriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Display

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Differential Display Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 85))

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Abstract

Since many life processes, such as development and reproduction, depend on differential gene expression, the spatiotemporal pattern of gene expression is of prime biological interest. Accordingly, the concepts of the mRNA differential display (DD) and RNA fingerprinting techniques (1,2) promised a breakthrough in molecular biology, because they circumvented some severe limitations of traditional methods, such as differential screening and subtractive hybridization, during the analysis of differential gene expression.

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References

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© 1997 Humana Press Inc.

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Theißen, G., Fischer, A. (1997). RC4D—Restriction Fragment Length Polymorphism-Coupled Domain-Directed Differential Display. In: Liang, P., Pardee, A.B. (eds) Differential Display Methods and Protocols. Methods in Molecular Biology, vol 85. Humana Press. https://doi.org/10.1385/0-89603-489-5:123

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  • DOI: https://doi.org/10.1385/0-89603-489-5:123

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-489-1

  • Online ISBN: 978-1-59259-569-3

  • eBook Packages: Springer Protocols

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