Partitioning of Proteins in Triton X-114

  • James G. Pryde
Part of the Methods in Molecular Biology™ book series (MIMB, volume 88)


Cultured mammalian cells and tissues can be solubilized by the nonionic detergent Triton X-114 (octylphenoxy polyethoxyethanol). This detergent, when in solution above its critical micelle concentration, increases its micelle weight when warmed from 0 to 20°C and in the process decreases its critical micelle concentration. This induces intermicellar interactions, which lead to turbidity (the cloud point) and phase separation of the detergent at 20°C. A simple low-speed centrifugation step recovers the detergent-enriched phase as an oily pellet. Following solubilization and warming to 20°C, integral membrane proteins partition into the detergent-enriched phase, and peripheral and cytosolic proteins are recovered from the detergent-depleted aqueous phase.


Critical Micelle Concentration Integral Membrane Protein Chromaffin Granule Immunoprecipitation Buffer Cobalt Nitrate Hexahydrate 
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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • James G. Pryde
    • 1
  1. 1.Respiratory Medicine Unit, Rayne Laboratory, Department of Medicine (RIE)University of Edinburgh Medical SchoolEdinburghUK

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