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Cloning cDNA Ends Using RACE

  • Protocol
Molecular Diagnosis of Infectious Diseases

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 13))

Abstract

Most attempts to identify and isolate a novel cDNA result in the acquisition of clones that represent only a part of the mRNA’s complete sequence. The approach described here to clone the missing sequence (cDNA ends) employs polymerase chain reaction (PCR). Since the initial reports of rapid amplification of cDNA ends (RACE) (1) or related techniques (2,3), many labs have developed significant improvements on the basic approach (5–18). The most recent hybrid version of the relatively simple classic RACE is described here, as well as a more powerful but technically more challenging “New RACE” protocol which is adapted from the work of a number of laboratories (19–26). Commercial RACE kits are available from Bethesda Research Labortories (BRL; Gaithersburg, MD) (11) and Clontech (Palo Alto, CA) that are convenient but not as powerful as the most recent versions of Classic and New RACE.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, University Medical Center at Stony Brook, NY

    Yue Zhang & Michael A. Frohman

Authors
  1. Yue Zhang
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  2. Michael A. Frohman
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Editors and Affiliations

  1. University of Regensburg, Germany

    Udo Reischl

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© 1998 Humana Press Inc., Totowa, NJ

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Zhang, Y., Frohman, M.A. (1998). Cloning cDNA Ends Using RACE. In: Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 13. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-485-2:81

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  • DOI: https://doi.org/10.1385/0-89603-485-2:81

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-485-3

  • Online ISBN: 978-1-59259-597-6

  • eBook Packages: Springer Protocols

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