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The Quartz Crystal Microbalance (QCM) as an Immunosensor

  • Franz Aberl
  • Conrad Kößlinger
  • Hans Wolf
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 13)

Abstract

This chapter demonstrates a preliminary experimental approach to detect antiviral antibodies by means of a quartz crystal immunosensor. As an example of the large numbers of immunoassays currently applied in the clinical laboratory, the screening for human immunodeficiency virus (HIV) specific antibodies was presented as a model application. The quartz crystal microbalance belongs to the group of acoustic immunosensors and its principle of operation is based on the propagation of acoustic shear waves in the substrate of the sensor. Phase and velocity of the acoustic wave are influenced by the specific adsorption of antibody molecules onto the antigen-coated sensor surface. This primary measuring allows the continuous monitoring of molecular interactions in liquids.

Keywords

Resonant Frequency Quartz Crystal Quartz Crystal Microbalance Sensor Surface Antiviral Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Sauerbrey G. (1959) Verwendung von Schwingquarzen zur Wagung dunner Schichten und zur Mikrowagung Zeztschrift fur Physik 155, 206–222CrossRefGoogle Scholar
  2. 2.
    Shons A, Dorman F, and Najarian J. (1972) An immunospecific microbalance J Biomed Mater Res 6, 565–570.PubMedCrossRefGoogle Scholar
  3. 3.
    Thompson M, Arthur C L, and Dhaliwal G K (1986) Liquid-phase piezoelectric and acoustic transmission studies of interfacial immunochemistry Anal Chem 58, 1206–1209PubMedCrossRefGoogle Scholar
  4. 4.
    Davis K A and Leary T R (1989) Continuous liquid-phase piezoelectrtc biosensor for kinetic immunoassays Anal Chem 61, 1227–1230PubMedCrossRefGoogle Scholar
  5. 5.
    Miura N, Higobashi H., Sakai G., Takeyuasu A, Uda T, and Yamazoe N (1993) Piezoelectric crystal immunosensor for sensitive detection of methamphetamine (stimulant drug) in human urine. Sensors and Actuators B 13-14, 188–191.CrossRefGoogle Scholar
  6. 6.
    Koßlinger C, Rehder A, Drost S, Sandmater H, Aberl F, Modrow S., and Wolf H (1991) A quartz crystal as an immunosensor Conf Proc Transduc 91, 693–696Google Scholar
  7. 7.
    Koßlinger C, Drost S, Aberl F, and Wolf H. (1994) Quartz crystal microbalante for immunosensing Fresenius J. Anal Chem 349, 349-345Google Scholar
  8. 8.
    Aberl F., Wolf H., Koßlinger C., Drost S, Woias P., and Koch S. (1994) HIV serology using piezoelectric immunosensors. Sensors and Actuators 18-19, 271–275Google Scholar
  9. 9.
    Kanazawa K. K. and Gordon J. G., II (1985) The oscillation frequency of a quartz resonator in contact with a liquid Anal Chem. Acta 175, 99–106.CrossRefGoogle Scholar
  10. 10.
    Muramatsu H, Tamiya E., and Karube I (1988) Computation of equivalent circuit parameters of quartz crystals in contact with liquids and study of liquid properties Anal Chem 60, 2142–2146CrossRefGoogle Scholar
  11. 11.
    Vornhagen R., Hinderer W., Nebel-Schickel H, Horn J, Pichler W., Franke M., Wolf H., and Sonneborn H.-H. (1990) Development of efficient HIV-spe-cific test systems using recombinant viral antigens Biotest Bull. 4, 91–96.Google Scholar
  12. 12.
    Ruzicka J. and Hansen E. H. (1988) Flow Injection Analysis, 2nd ed. Wiley, New York.Google Scholar
  13. 13.
    Horrisberger M. and Vauthey M. (1984) Labelling of colloidal gold with protein Histochemistry 80, 111–118Google Scholar
  14. 14.
    Williams R L and Williams D. F. (1988) The effect of albumin on the wettability of pure metal and metal oxide surfaces. J Colloid Interface Sci 126, 596–603CrossRefGoogle Scholar
  15. 15.
    Abetl F. (1994) Ionensenstitive Feldeffekttransistoren und piezoelectrische Sensoren als Meßwandler fur die Erkennungsreaktion zwischen Antigen-und Antikorpermolekulen, Thesis, Tech Univ Munich, GermanyGoogle Scholar

Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Franz Aberl
    • 1
  • Conrad Kößlinger
    • 1
  • Hans Wolf
    • 2
  1. 1.SecuretecMunichGermany
  2. 2.Institute for Medical Microbiology and HygieneUniversity of RegensburgGermany

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