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Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung

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Gene Therapy Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 7))

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Abstract

With the advent of improved vectors for DNA delivery, somatic gene therapeutic approaches have expanded rapidly in the last few years. The vast majority of applications include ex vivo and in vivo protocols in patients postnatally. Nonetheless there is increasing interest and compelling reasons to consider prenatal application of somatic gene therapy (1,2. In the current chapter, we will review theoretical, ethical, and experimental support for in utero gene therapy and then outline the methodology and large animal model we are currently using to consider retrovirus-mediated gene transfer to fetal lung. In this latter regard, the candidate inherited disorder is cystic fibrosis and the reader is referred to Chapters 1 and 12 in this volume.

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Author information

Authors and Affiliations

  1. Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Bruce R. Pitt

  2. Babies Hospital, Columbia University College of Physicians and Surgeons, New York, NY, USA

    Margaret A. Schwarz

  3. Division of Lung Biology, Childrens Research Center University of Utah School of Medicine, Salt Lake City, UT, USA

    Richard D. Bland

Authors
  1. Bruce R. Pitt
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  2. Margaret A. Schwarz
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  3. Richard D. Bland
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Editor information

Editors and Affiliations

  1. University of Pittsburgh, PA, USA

    Paul D. Robbins

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© 1997 Humana Press Inc., Totowa, NJ

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Pitt, B.R., Schwarz, M.A., Bland, R.D. (1997). Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung. In: Robbins, P.D. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 7. Humana, Totowa, NJ. https://doi.org/10.1385/0-89603-484-4:185

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  • DOI: https://doi.org/10.1385/0-89603-484-4:185

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-484-6

  • Online ISBN: 978-1-59259-591-4

  • eBook Packages: Springer Protocols

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