Abstract
The use of degenerate primers in the polymerase chain reaction (PCR) is an effective method for identifying related genes that share limited sequence similarity. Other methods, using oligonucleotide or heterologous probes, for example, may fail to identify genes that are not highly conserved, have diverged over evolutionary time, or share only short regions of structural similarity.
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© 1997 Humana Press Inc.
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Bartl, S. (1997). Amplification Using Degenerate Primers with Multiple Inosines to Isolate Genes with Minimal Sequence Similarity. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:451
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DOI: https://doi.org/10.1385/0-89603-483-6:451
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-483-9
Online ISBN: 978-1-59259-553-2
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