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Subtractive cDNA Cloning Using Magnetic Beads and PCR

  • Protocol
PCR Cloning Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 67))

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Abstract

Subtractive cloning is a method that facilitates the isolation of nucleotide sequences present in a test sample but absent or present at much lower levels, in a reference sample. A variety of situations lend themselves to this methodology and it has been used, for example, to characterize changes in levels of gene expression during development (1–3), in pathological situations (4–6), in inductive events (7,8), and for the isolation of tissue-specific genes (1,9–11). Current methodologies also allow detection of pathogens and integrated retroviruses present at exceedingly low levels in the cell (12).

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References

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Author information

Authors and Affiliations

  1. Organogenesis Lab, University of Oxford, UK

    Thierry G. Coche

Authors
  1. Thierry G. Coche
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Editor information

Editors and Affiliations

  1. Department of Anatomy, University of Connecticut Health Center, Farmington, CT

    Bruce A. White

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© 1997 Humana Press Inc.

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Coche, T.G. (1997). Subtractive cDNA Cloning Using Magnetic Beads and PCR. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:371

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  • DOI: https://doi.org/10.1385/0-89603-483-6:371

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-483-9

  • Online ISBN: 978-1-59259-553-2

  • eBook Packages: Springer Protocols

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