Abstract
Oligonucleotide-directed mutagenesis techniques are extensively used for studying gene regulation and DNA and protein structure/function relationships. A number of PCR-based mutagenesis methods have been developed recently (1–8). In general, these PCR-based approaches are simpler and faster than classical mutagenesis methods employing in vivo DNA synthesis, and several of them are now widely practiced.
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© 1997 Humana Press Inc.
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Picard, V., Bock, S.C. (1997). Rapid and Efficient One-Tube PCR-Based Mutagenesis Method. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:183
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DOI: https://doi.org/10.1385/0-89603-483-6:183
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-483-9
Online ISBN: 978-1-59259-553-2
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