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Recombination and Site-Directed Mutagenesis Using Recombination PCR

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PCR Cloning Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 67))

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Abstract

The polymerase chain reaction (PCR) (1) provides a rapid means for the recombination and site-directed mutagenesis of DNA (2). DNA modification can occur during PCR because the primers are incorporated into the ends of the PCR product. The simplest PCR-based method for site-directed mutagenesis and DNA recombination is recombination PCR.

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Author information

Authors and Affiliations

  1. Department of Pediatrics, University of Iowa, Iowa City, IA

    Douglas H. Jones & Stanley C. Winistorfer

Authors
  1. Douglas H. Jones
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  2. Stanley C. Winistorfer
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Editor information

Editors and Affiliations

  1. Department of Anatomy, University of Connecticut Health Center, Farmington, CT

    Bruce A. White

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© 1997 Humana Press Inc.

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Jones, D.H., Winistorfer, S.C. (1997). Recombination and Site-Directed Mutagenesis Using Recombination PCR. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:131

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  • DOI: https://doi.org/10.1385/0-89603-483-6:131

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-483-9

  • Online ISBN: 978-1-59259-553-2

  • eBook Packages: Springer Protocols

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