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Exon Detection by Similarity Searches

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Gene Isolation and Mapping Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 68))

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Abstract

Other chapters of this volume have presented the various experimental methods (mainly exon trapping and recombination-based and hybridization-based approaches) used for the identification of transcribed sequences within cloned genomic fragments. None of those methods require detailed sequence information on the genomic region of interest. However, since generating large genomic sequences is becoming more routine, identifying transcribed regions by computer analysis of large genomic sequence (i.e., “software trapping”) is also becoming a viable alternative. After an overview of the various computational methods at hand, this chapter focuses on the use of database similarity searches for the identification of exons in mammalian genomes.

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Authors and Affiliations

  1. Institut de Biologie Structurale et Microbiologie, Marseille, France

    Jean-Michel Claverie

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  1. Jean-Michel Claverie
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Editors and Affiliations

  1. John Radcliffe Hospital, University of Oxford, UK

    Jacqueline Boultwood

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© 1997 Humana Press Inc., Totowa, NJ

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Claverie, JM. (1997). Exon Detection by Similarity Searches. In: Boultwood, J. (eds) Gene Isolation and Mapping Protocols. Methods in Molecular Biology™, vol 68. Humana Press. https://doi.org/10.1385/0-89603-482-8:283

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  • DOI: https://doi.org/10.1385/0-89603-482-8:283

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-482-2

  • Online ISBN: 978-1-59259-554-9

  • eBook Packages: Springer Protocols

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