Abstract
Reporter gene assays have become essential tools for the study of gene regulation. Several genes have been adapted as indicators of transcriptional activity with a variety of detection techniques including radioisotopic, colorimetric, luminescent, fluorescent, and immunoassay methods (1–4). Chemiluminescent assays have been very effective in detecting enzyme labels in direct enzyme activity assays, immunoassays, and nucleic acid hybridization assays.
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© 1997 Humana Press Inc., Totowa, NJ
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Olesen, C.E.M., Fortin, J.J., Voyta, J.C., Bronstein, I. (1997). Detection of β-Galactosidase and β-Glucuronidase Using Chemiluminescent Reporter Gene Assays. In: Tuan, R.S. (eds) Recombinant Protein Protocols. Methods in Molecular Biology™, vol 63. Humana Press. https://doi.org/10.1385/0-89603-481-X:61
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DOI: https://doi.org/10.1385/0-89603-481-X:61
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