Abstract
The spatio-temporal study of gene expression in tissues is typically accomplished by using one of the following techniques: in situ hybridization (ISH), that localizes mRNA transcripts in particular cell populations using a labeled nucleic acid probe; and immunohistochemistry (IMH), that detects protein gene products via labeled primary or secondary antibodies. Often, ISH and IMH are performed separately on adjacent serial sections, allowing comparison of the cellular staining pattern for mRNA, and the presence or absence of the corresponding protein product. Combining these two procedures, by performing them on the same tissue section provides immediate, direct visualization of the expression pattern of a particular gene, avoiding the need to visually interpret and compare two separate sets of data.
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© 1997 Humana Press Inc., Totowa, NJ
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Shepley, K.J., Tuan, R.S. (1997). Combined, Sequential In Situ Hybridization and Immunohistochemistry on the Same Tissue Section. In: Tuan, R.S. (eds) Recombinant Protein Protocols. Methods in Molecular Biology™, vol 63. Humana Press. https://doi.org/10.1385/0-89603-481-X:247
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DOI: https://doi.org/10.1385/0-89603-481-X:247
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