Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growth Hormone Reporter Proteins

  • Corinne E. M. Olesen
  • John C. Voyta
  • Irena Bronstein
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 63)

Abstract

Chemiluminescent substrates have been successfully used for highly sensitive quantitation of reporter enzyme activity (see  Chapters 5 and  6) and in enzyme-linked immunoassays for highly sensitive detection of many analytes (1, 2, 3, 4, 5, 6). CSPD chemiluminescent 1,2-dioxetane substrate (7) for alkaline phosphatase has been used with Sapphire-II enhancing reagent for enzyme-linked immunoassay detection of chloramphenicol acetyltransferase and human growth hormone reporter gene products. These chemiluminescent reporter gene assay systems provide nonradioactive, simple, sensitive detection methods, performed in microplate or tube luminometers.

Keywords

Microwave EDTA Rubber Fluoride MgCl2 

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Copyright information

© Humana Press Inc., Totowa, NJ 1997

Authors and Affiliations

  • Corinne E. M. Olesen
    • 1
  • John C. Voyta
    • 1
  • Irena Bronstein
    • 1
  1. 1.TropixBedford

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