Use of Gonadal Primordial Germ Cells (PGCs) as Tools for Gene Transfer in Chickens

  • Nathalie Allioli
  • Gérard Verdier
  • Catherine Legras
Part of the Methods in Molecular Biology book series (MIMB, volume 62)

Abstract

As a result of morphological and developmental characteristics of the early avian embryo, several approaches, taking place at early stages, have been investigated to introduce foreign DNA into the avian genome. They include:
  1. 1.

    Insemination with sperm cells to which foreign DNA was coated (1,2);

     
  2. 2.

    Transfection of DNA into follicles before fertilization (3),

     
  3. 3.

    Direct injection of naked DNA into newly fertilized ova or into germinal disk (4, 5, 6, 7),

     
  4. 4.

    Microinjection of replication-defective retroviral vectors (8-10) into unincubated embryo blastoderm, and;

     
  5. 5.

    Retroviral infection of primordial germ cells, PGCs (11,12).

     

Keywords

Microwave DMSO Penicillin Germinal Dimethyl 

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Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • Nathalie Allioli
    • 1
  • Gérard Verdier
    • 1
  • Catherine Legras
    • 1
  1. 1.Centre de Genetique Moleculaire et CellularieUniversite Claude Bernard Lyon I, VillcurbanneCedexFrance

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