Experimental Strategies in Efficient Transfection of Mammalian Cells

Calcium Phosphate and DEAE-Dextran
  • Gregory S. Pari
  • Wayne A. Keown
Part of the Methods in Molecular Biology book series (MIMB, volume 62)


Transfection of foreign DNA into mammalian cells is one of the most common procedures performed by investigators next to cell culture itself. Whether the goal is to express mammalian or viral genes transiently or by the generation of stable cells lines, researchers have a variety of transfection methods to choose from Calcium phosphate coprecipitation and DEAE-dextran-mediated transfection are the oldest, and with many investigators, the most trusted of these methods. Paradoxically, the exact mechanism of DNA uptake for each of these methods remains unknown.


Calcium Phosphate Express Cell Line Calcium Phosphate Method Effector Plasmid Calcium Phosphate Transfection 
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Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • Gregory S. Pari
    • 1
  • Wayne A. Keown
    • 1
  1. 1.HybridonWorcester

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