Abstract
Nucleic acid sequence-based amplification (NASBA) is a primer dependent, homogeneous, isothermal amplification process for the detection of RNA (1). It can result in 109- fold amplification of a specific RNA sequence and offers the unique possibility to amplify RNA in a background of genomic DNA. Detection of RNA can be used to monitor gene expression or cell viability or can be a prognostic indication for virus replication and production. Amplification of RNA beyond the detection limit is easily achieved because of the often high copy numbers of RNA targets in a cell. NASBA already has been used for the detection of human immunodeficiency virus (HIV) (2–11) hepatitis C virus (HCV) (12,13) and cytomegalovirus (CMV).
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© 1998 Humana Press Inc.
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van Strijp, D., van Aarle, P. (1998). NASBA. In: Stephenson, J.R., Warnes, A. (eds) Diagnostic Virology Protocols. Methods in Molecular Medicine™, vol 12. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-479-8:331
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DOI: https://doi.org/10.1385/0-89603-479-8:331
Publisher Name: Springer, Totowa, NJ
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