Abstract
In 1953, the first adenovirus was isolated from a human and, subsequently, 47 types have been shown to exist. Adenoviruses are now classified into six subgroups (A–F), which are based on their hemagglutination properties (1). They have a icosahedron structure that contains double-stranded linear DNA of 45,000 basepairs. Although, adenoviruses can cause a range of infections in humans, including conjunctivitis, pharyngitis, and gastroenteritis, this chapter focuses on gastroenteric adenoviruses and their rapid detection in fecal samples.
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References
Hierholzer, J C (1991) Antigenic relationships among the 47 human adenoviruses determined in reference horse sera. Arch Virol 121, 179–197.
Albert, M J (1986) Enteric adenoviruses. Arch Virol 88, 1–17.
Cepko, C. L., Whetstone, C. A, and Sharp, P. A. (1983) Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. J Clin. Microbiol 17(2), 360–364.
Grydsuk, J. D., Fortsas, E, Petric, M, and Brown, M. (1996) Common epitope on protein VI of enteric adenoviruses from subgenera A and F. J Gen Virol 77(8), 18l–l89.
Bhan, M. K., Raj, P., Bhandari, N., Svensson, L., Stintzing, G., Prasad, A. K., Jayashree, S., and Srivastava, R. (1988) Role of enteric adenoviruses and rotaviruses in mild and severe acute enteritis. Pediatr Infect Dis. J 7(5), 320–323.
Petric, M (1995) Caliciviruses, astroviruses and other diarrheic viruses, in Manual of Clinical Microbiology, 6th ed. (Murray, P. R, Baron, E. J., Pfaller, M. A, Tenover, F. C, and Yolken, R. H., eds.), ASM, Washington, DC, pp. 1017–1024.
Noel, J, Mansoor, A., Thaker, U, Herrmann, J., Perron-Henry, D, and Cubitt, W D. (1994) Identification of adenoviruses in faeces from patients with diarrhoea at the Hospitals for Sick Children, London, 1989-l992. J,Med Viro 43(l), 84–90.
Grandien, M., Pettersson, C. A., Svensson, L., and Uhnoo, I (1987) Latex agglutination test for adenovirus diagnosis in diarrheal disease .J Med Virol 23(4), 311–316.
Doonan, S. (ed.) (1996) Methods in Molecular Biology Protein Purification Protocols. Humana, Totowa, NJ.
Sanekata, T., Yoshida, Y, and Okada, H. (1981) Detection of rotavirus in faeces by latex agglutination. J Immunol Methods 41, 377–385.
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© 1998 Humana Press Inc.
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Tabish, T., Warnes, A., Clark, S. (1998). Rapid Detection of Adenovirus from Fecal Specimens. In: Stephenson, J.R., Warnes, A. (eds) Diagnostic Virology Protocols. Methods in Molecular Medicine™, vol 12. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-479-8:1
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DOI: https://doi.org/10.1385/0-89603-479-8:1
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-479-2
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