Skip to main content

Rapid Detection of Adenovirus from Fecal Specimens

  • Protocol
Diagnostic Virology Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 12))

  • 574 Accesses

Abstract

In 1953, the first adenovirus was isolated from a human and, subsequently, 47 types have been shown to exist. Adenoviruses are now classified into six subgroups (A–F), which are based on their hemagglutination properties (1). They have a icosahedron structure that contains double-stranded linear DNA of 45,000 basepairs. Although, adenoviruses can cause a range of infections in humans, including conjunctivitis, pharyngitis, and gastroenteritis, this chapter focuses on gastroenteric adenoviruses and their rapid detection in fecal samples.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Hierholzer, J C (1991) Antigenic relationships among the 47 human adenoviruses determined in reference horse sera. Arch Virol 121, 179–197.

    Article  PubMed  CAS  Google Scholar 

  2. Albert, M J (1986) Enteric adenoviruses. Arch Virol 88, 1–17.

    Article  PubMed  CAS  Google Scholar 

  3. Cepko, C. L., Whetstone, C. A, and Sharp, P. A. (1983) Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. J Clin. Microbiol 17(2), 360–364.

    PubMed  CAS  Google Scholar 

  4. Grydsuk, J. D., Fortsas, E, Petric, M, and Brown, M. (1996) Common epitope on protein VI of enteric adenoviruses from subgenera A and F. J Gen Virol 77(8), 18l–l89.

    Article  Google Scholar 

  5. Bhan, M. K., Raj, P., Bhandari, N., Svensson, L., Stintzing, G., Prasad, A. K., Jayashree, S., and Srivastava, R. (1988) Role of enteric adenoviruses and rotaviruses in mild and severe acute enteritis. Pediatr Infect Dis. J 7(5), 320–323.

    Article  PubMed  CAS  Google Scholar 

  6. Petric, M (1995) Caliciviruses, astroviruses and other diarrheic viruses, in Manual of Clinical Microbiology, 6th ed. (Murray, P. R, Baron, E. J., Pfaller, M. A, Tenover, F. C, and Yolken, R. H., eds.), ASM, Washington, DC, pp. 1017–1024.

    Google Scholar 

  7. Noel, J, Mansoor, A., Thaker, U, Herrmann, J., Perron-Henry, D, and Cubitt, W D. (1994) Identification of adenoviruses in faeces from patients with diarrhoea at the Hospitals for Sick Children, London, 1989-l992. J,Med Viro 43(l), 84–90.

    Article  CAS  Google Scholar 

  8. Grandien, M., Pettersson, C. A., Svensson, L., and Uhnoo, I (1987) Latex agglutination test for adenovirus diagnosis in diarrheal disease .J Med Virol 23(4), 311–316.

    Article  PubMed  CAS  Google Scholar 

  9. Doonan, S. (ed.) (1996) Methods in Molecular Biology Protein Purification Protocols. Humana, Totowa, NJ.

    Google Scholar 

  10. Sanekata, T., Yoshida, Y, and Okada, H. (1981) Detection of rotavirus in faeces by latex agglutination. J Immunol Methods 41, 377–385.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc.

About this protocol

Cite this protocol

Tabish, T., Warnes, A., Clark, S. (1998). Rapid Detection of Adenovirus from Fecal Specimens. In: Stephenson, J.R., Warnes, A. (eds) Diagnostic Virology Protocols. Methods in Molecular Medicine™, vol 12. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-479-8:1

Download citation

  • DOI: https://doi.org/10.1385/0-89603-479-8:1

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-479-2

  • Online ISBN: 978-1-59259-596-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics