A PCR-Based Approach to Hammerhead Ribozyme Synthesis

  • Katalin Karikó
  • Elliot S. Barnathan
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 11)

Abstract

Inactivation of gene expression by antisense mechanisms in general and by ribozymes in particular show great promise as a research tool as well as therapeutic agent in selectively reducing mRNA species and thus decreasing subsequent protein synthesis (for reviews see refs. 1 and 2).

Keywords

Vortex Phenol Chloroform Glycol Electrophoresis 

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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Katalin Karikó
    • 1
  • Elliot S. Barnathan
    • 2
  1. 1.Neurosurgery and Cardiovascular DivisionUniversity of Pennsylvania School of MedicinePhiladelphia
  2. 2.Cardiovascular DivisionUniversity of PennsylvaniaPhiladelphia

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