Ribozymes Targeted Against AML1/MTG8

Evaluation of Ribozyme Efficiency in a Cell-Free System and Transfection with Cationic Liposome
  • Hiromichi Matsushita
  • Hiroyuki Kobayashi
  • Masahiro Kizaki
  • Yasuo Ikeda
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 11)


It is often found that tumors have chromosomal translocation, especially in hematological malignancies. A specific chromosomal translocation is related to a specific type of disease. Recent studies have revealed that genetic aberrations originated from these chromosomal translocations have a key role in oncogenesis. Formation of a chimeric fusion gene is one such genetic aberration. Because chimeric fusion genes are expressed specifically in tumor cells and participate in oncogenesis, specific gene inhibition of the chimeric fusion genes is thought to be a good approach for treatment.


Cationic Liposome Cleavage Reaction Hammerhead Ribozyme Reduce Serum Medium Saran Wrap 
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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Hiromichi Matsushita
    • 1
  • Hiroyuki Kobayashi
    • 2
  • Masahiro Kizaki
    • 3
  • Yasuo Ikeda
    • 3
  1. 1.Division of HematologyKeio University School of MedicineTokyoJapan
  2. 2.Department of Laboratory Medicine National Defense CollegeTokorozawa SaitamaJapan
  3. 3.Division of HematologyKeio University School of MedicineTokyoJapan

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