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Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid

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Free Radical and Antioxidant Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 108))

Abstract

Reactive-oxygen species and organic free radicals are essential metabolic intermediates and have important regulatory functions. Overproduction of these reactive metabolites is implicated in the etiology of a host of degenerative diseases (1), such as cardiovascular disease and neurodegenerative disease, in acute conditions such as trauma and infection, and in aging. Of the various types of oxidative damage that cells undergo, lipid peroxidation is considered to be one of the major contributors to oxidative injury (2); thus, strict regulation of lipid peroxidation is extremely important for normal cell physiology. Quantitative assays of lipid peroxidation in intact cells are essential for evaluating oxidative damage from various sources, and in testing the efficacy of antioxidant interventions.

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Author information

Authors and Affiliations

  1. Department of Environmental and Occupational Health, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA

    Valerian E. Kagan & Vladimir B. Ritov

  2. Department of Environmental and Occupational Health, University of Pittsburgh, PA

    Yulia Y. Tyurina & Vladimir A. Tyurin

Authors
  1. Valerian E. Kagan
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  2. Vladimir B. Ritov
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  3. Yulia Y. Tyurina
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  4. Vladimir A. Tyurin
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Editor information

Editors and Affiliations

  1. State University of New York, Buffalo, NY

    Donald Armstrong

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© 1998 Humana Press Inc., Totowa, NJ

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Kagan, V.E., Ritov, V.B., Tyurina, Y.Y., Tyurin, V.A. (1998). Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:71

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  • DOI: https://doi.org/10.1385/0-89603-472-0:71

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-472-3

  • Online ISBN: 978-1-59259-254-8

  • eBook Packages: Springer Protocols

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