Abstracct
Lipid hydroperoxides (LOOH) are the primary products of lipid peroxidation. Therefore, it would be desirable to detect and identify the lipid hydroperoxides in tissues or blood. One of the most advanced methods for the detection of LOOH is a chemiluminescence-based high-performance liquid chromatography (HPLC) assay (1–5) as shown in Fig. 1. The reaction sequence leading to the emission of light from isoluminol in the presence of LOOH and microperoxidase, a heme catalyst, is assumed to be as follows:
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Yamamoto, Y., Brodsky, M. H., Baker, J. C., and Ames, B. N. (1987) Detection and characterization of lipid hydroperoxides at picomole levels by high-performance liquid chromatography. Anal. Biochem. 160,7–13
Yamamoto, Y and Ames, B N. (1987) Detection of lipid hydroperoxides and hydrogen peroxide at picomole levels by an HPLC and isoluminol chemiluminescence assay. Free Rad. Biol. Med.. 3, 359–361.
Frei, B., Yamamoto, Y., Niclas, D., and Ames, B. N. (1988) Evaluation of an isoluminol chemiluminescence assay for the detection of hydroperoxides in human blood plasma. Anal. Biochem. 175, 120–130.
Yamamoto, Y., Frei, B., and Ames, B. N. (1990) Assay of lipid hydroperoxides using HPLC with post-column chemiluminescence detection Methods Enzymol 186, 371–380.
Yamamoto, Y (1994) Chemiluminescence-based high-performance liquid chromatography assay of lipid hydroperoxides Methods Enzymol 233, 319–324
Yamamoto, Y and Niki, E. (1989) Presence of cholesteryl ester hydroperoxide in human blood plasma. Biochem Biophys. Res. Commun. 165, 988–993.
Yamamoto, Y, Wakabayashi, K, Niki, E., and Nagao, M. (1992) Comparison of plasma levels of lipid hydroperoxides and antioxidants in hyperhpidemic Nagase analbuminemic rats, Sprague-Dawley rats, and humans. Biochem. Biophys. Res. Commun. 189, 518–523.
Yamamoto, Y., Nagata, Y, Niki, E., Watanabe, K., and Yoshimura, S. (1993) Plasma glutathione peroxidase reduces phosphatidylcholine hydroperoxide Biochem. Biophys Res. Commun 193, 133–138.
Nagata, Y., Yamamoto, Y., and Niki, E (1996) Reaction of phosphatidylcholine hydroperoxide in human plasma: The role of peroxidase and lecithin:cholesterol acyltransferase Arch Biochem. Biophys. 329, 24–30.
Bowry, V. W. and Stocker, R. (1993) Tocopherol-mediated peroxidation. The prooxidant effect of vitamin E on the radical-initiated oxidation of human low-density lipoprotein. J. Am. Chem. Soc. 115, 6029–6044.
Chan, H. W.-S. and Levett, G. (1977) Autoxidation of methyl linoleate Separation and analysis of isomeric mixtures of methyl linoleate hydroperoxides and methyl hydroxylinoleates Lipids 12, 99–104.
Nakamura, Y, Ohori, Y., and Yamamoto, Y. (1992) Formation of phosphatidylcholine hydroperoxide in isolated rat hepatocytes treated with tert-butyl hydroperoxide, in Oxygen Radicals Proceedings of the 5th International Congress on Oxygen Radicals (Yagi, K., Kondo, M., Niki, E., and Yoshikawa, T., eds.), Excerta Medica, Amsterdam, pp 273–276.
Kambayashi, Y., Yamashita, S., Niki, E., and Yamamoto, Y. (1997) Oxidation of rat liver phospholipids: comparison of pathways in homogeneous solution, in liposomal suspension and in whole tissue homogenates J Biochem. 121, 425–431
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Yamamoto, Y., Kambayashi, Y., Ueda, T. (1998). Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:63
Download citation
DOI: https://doi.org/10.1385/0-89603-472-0:63
Publisher Name: Humana Press
Print ISBN: 978-0-89603-472-3
Online ISBN: 978-1-59259-254-8
eBook Packages: Springer Protocols