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Free Radical and Antioxidant Protocols pp 57–62Cite as

Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 108))

Abstract

Free radicals generated by a wide variety of processes, such as ionizing radiation (1), toxic xenobiotics (2), inflammation (3), and metabolites of membrane lipid transformation (4), are involved in various diseases (5–9). A number of disorders in cellular and organ systems have been attributed to them. Free radicals damage lipids (7), proteins (10) and deoxyribonucleic acid (DNA) (11,12) with consequent effects ranging from cell death to neoplasia (13). It is therefore important to measure the amount of free radicals generated by varrous cell and organ types. Traditionally, one would measure free-radical generation by electron spin resonance, which is costly or complicated, or measure antioxidants that scavenge free radicals to get an estimate of the oxidative state.

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Author information

Authors and Affiliations

  1. Vascular Lab, Endocrinology-Diabetes Center of Western New York, Millard Fillmore Health System, Buffalo, NY

    Kuldip Thusu

  2. Molecular Biology Laboratory, Endocrine-Diabetes Center of Western New York, Millard Fillmore Health System, Buffalo, NY

    Ehad Abdel-Rahman

  3. Department of Medicine, University of Buffalo School of Medicine and Biomedical Sciences, Endocrinology-Diabetes Center of Western New York, Millard Fillmore Hospital, Buffalo, NY

    Paresh Dandona

Authors
  1. Kuldip Thusu
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  2. Ehad Abdel-Rahman
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  3. Paresh Dandona
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Editor information

Editors and Affiliations

  1. State University of New York, Buffalo, NY

    Donald Armstrong

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© 1998 Humana Press Inc., Totowa, NJ

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Thusu, K., Abdel-Rahman, E., Dandona, P. (1998). Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:57

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  • DOI: https://doi.org/10.1385/0-89603-472-0:57

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-472-3

  • Online ISBN: 978-1-59259-254-8

  • eBook Packages: Springer Protocols

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