Skip to main content
SpringerLink
Log in

Purification of Vesicular Carriers from Rat Hepatocytes by Magnetic Immunoadsorbtion

  • Protocol
Book cover Free Radical and Antioxidant Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 108))

  • 1505 Accesses

Abstract

Vesicular carriers represent a cellular entity involved in the transfer of proteins between different intracellular compartments. They were first identified and described by Jamieson and Palade (1) in their studies on the process of protein secretion in guinea-pig pancreas. Most of the evidence obtained since then is in favor of the vesicular nature of the intracellular transport (2,3).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Palade, G. E (1975) Intracellular aspects of the process of protein (synthesis) secretion. Science 89, 347–358

    Article  Google Scholar 

  2. Rothman, J. E. (1994) Mechanisms of intracellular protein transport. Nature 372, 55–63

    Article  PubMed  CAS  Google Scholar 

  3. Pryer, K. N., Wuestehube, L. J., and Schekman, R. (1992) Vesicle-mediated protein sorting Annu. Rev. Biochem. 61, 471–516

    Article  PubMed  CAS  Google Scholar 

  4. Pfeffer, S. R. and Rothman, J. E. (1987) Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi. Annu Rev. Biochem. 56, 829–852.

    Article  PubMed  CAS  Google Scholar 

  5. Beaufay, H. and Amar-Costesec, A. (1976) Cell fractionation techniques Methods Membr. Biol. 6, 1–99

    CAS  Google Scholar 

  6. Howell, K. E., Schmid, R., Ugelstad, J., and Gruenberg, J. (1989) Immunoisolation using magnetic solid supports: subcellular fractionation for cell-free functional studies, in Laboratory Methods in Vesicular and Vectorial Transport (Tartakoff, A., ed.), Academic Press, Inc, New York, pp 171–198

    Google Scholar 

  7. Ugelstad, J., Mork, P. C., Kaggerud, K. H., Ellmgsen, T., and Berge, A. (1980) Swelling of oligomer-polymer perticles new methods of preparation of emulsions and polymer dispersions. Adv Colloid Interface Sci. 13, 1201–1243

    Article  Google Scholar 

  8. Ugelstad, J., Kilaas, L., Aune, O., Biorgum, J., Herje, R., Schmid, R, Stenstad, P., and Berge, A. (1994) Monodisperse polymer particles: preparation and new biochemical and biomedical applications, in Advances in Biomagnetic Separation (Uhlen, M, Homes, E, and Olsvik, 0., eds), Eaton Publishing Co., Natick, MA, pp. 1–19.

    Google Scholar 

  9. Saucan, L. and Palade, G. E (1994) Membrane and secretory proteins are transported from the Golgi complex to the sinusoidal plasmalemma of hepatocytes by distinct vesicular carriers J. Cell Biol. 125(4), 733–741

    Article  PubMed  CAS  Google Scholar 

  10. Nustad, K., Danielsen, H., Reith, A., Funderud, S., Lea, T., Vartdal, F., and Ugelstad, J. (1988) Monodisperse particles in immunoassays and cell separation, in Microspheres: Medical and Biological Applications (Rembaum, A. and Tokes, Z. A, eds), CRC Press, Boca Raton, FL, pp 53–75

    Google Scholar 

  11. Howell, K. E., Crosby, J. R., Ladinsky, M. S., Jones, S. M., Schmid, R., and Ugelstad, J. (1994) Magnetic solid supports for cell-free analysis of vesicular transport, in Advances in Biomagnetic Separation (Uhlen, M., Homes, E., and Olsvik, 0., eds), Eaton Publishing Co, Natick, MA, pp. 195–204

    Google Scholar 

  12. Luers, G., Hartig, R., Fahimi, H. D., Cremer, C, and Volkl, A. (1996) Immunomagnetic isolation of peroxisomes. Ann NY Acad Sci. 804, 698–700.

    Article  PubMed  CAS  Google Scholar 

  13. Kvalheim, G., Fodstad, G., Phil, A., Nustad, K., Pharo, A., Ugelstad, J., and Funderud, S. (1987) Elimination of B-Lymphoma cells from human bone marrow model experiments using monodisperse magnetic particles coated with primary monoclonal antibodies Cancer Res 47, 846–851

    PubMed  CAS  Google Scholar 

  14. Howell, K. E. and Palade, G. E. (1982) Heterogeneity of lipoprotein particles in hepatic Golgi fractions J Cell Biol 92, 833–845

    Article  PubMed  CAS  Google Scholar 

  15. Ghetie, V., Mota, G, and Sjoquist, J. (1978) Separation of cells by affinity chromatography on SpA-Sepharose 6MB. J Immunol Methods 21, 133–141.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Vascular Disease Prevention Laboratory, Carl T. Hayden VA Medical Center, Phoenix, AZ

    Lucian Saucan

Authors
  1. Lucian Saucan
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. State University of New York, Buffalo, NY

    Donald Armstrong

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Saucan, L. (1998). Purification of Vesicular Carriers from Rat Hepatocytes by Magnetic Immunoadsorbtion. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:257

Download citation

  • DOI: https://doi.org/10.1385/0-89603-472-0:257

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-472-3

  • Online ISBN: 978-1-59259-254-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation