Abstract
Autofluorescent ceroid/lipofuscin-type pigments are usually classified and defined as follows: Lipofuscin is an intracellular, age-related, fluorescent, cytoplasmic, granular pigment. It is mainly present in secondary lysosomes of post-mitotic cells, such as neurons, cardiac myocytes, and retinal pigment epithelial (RPE) cells Ceroid is a group of biopigments, also with an intralysosomal location, which are rapidly produced as a result of various pathologies and experimental conditions, such as x-irradiation, E-vitamin deficiency, starvation, and intoxication. Ceroid is probably akin to lipofuscin and may share the same mechanisms of formation, although it does not accumulate in relation to aging. It may be present in a variety of cells, e.g., in the kidney, thymus, pancreas, testis, prostate, seminal vesicles, uterus, and adrenal gland of man and animal (1–4). Advanced glycation end-products (AGEs) are mainly found extracellularly in association with long-lived proteins, e.g., in the cataractic lens of the eye (5,6), and in cross-linked collagen of the skin, arteries, lungs, and kidneys (7–9). They are formed during complex non-enzymatic glycation-reactions involving Maillard- and Amadori-type chemistry. AGEs may also, however, be present intracellularly, and they probably constitute a substantial part of lipofuscin and ceroid. A variety of synthetic age pigment-like fluorophores (APFs) can be produced by oxidation/peroxidation of different biological materials. Most such fluorophores are
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References
Strehler, B. L (ed.) (1977) Time, Cells, and Aging. 2nd ed. Academic Press, New York.
Ordy, J. M. and Brizzee, K. R (eds.) (1975) Neurobiology of Aging. Plenum Press, New York.
Feeney, L. (1978) Lipofuscin and melanin of human retinal pigment epithelium. Invest. Ophthal. Vis. Sci. 7, 583–600.
Miquel, J., Oro, J., Bensch, K. G., and Johnson, J. E. (1977) Lipofuscin fine-structural and biochemical studies, in Free Radicals in Biology Vol. III (Pryor, W. A., ed.), Academic Press, New York, pp. 133–182
Duncan, G. (ed.) (1981) Mechanisms of Cataract Formation in the Human Lens. Academic Press, New York
Monnier, V. M. and Cerami, A. (1983) Detection of nonenzymatic browning products in the human lens Biochim. Biophys. Acta 760, 97–103
Verzar, F. (1957) The ageing of connective tissue Gerontologia 1, 363–378
Monnier, V. M. and Cerami, A. (1983) Nonenzymatic glycosylation and browning of proteins in vivo, in The Maillard Reaction in Foods and Nutrition (Waller, G. R. and Feather, M S., eds.), American Chemical Society, Washington DC, pp. 431–149.
Baynes, J. W. and Monnier, V. M. (eds.) (1989) The Maillard Reaction in Aging, Diabetes, and Nutrition. Alan R. Liss, Inc., NY.
Strehler, B. L. (1964) On the histochemistry and ultrastructure of age pigment, in Advances in Gerontological Research (Strehler, B. L., ed.), Academic Press, New York, pp. 343–384.
Porta, E. A. and Hartroft, W. S. (1969) Lipid pigments in relation to aging and dietary factors, in Pigments in Pathology (Wolman, W., ed.), Academic Press, NY, pp. 191–235.
Sohal, R. (ed.) (1981) Age Pigments Elsevier, Amsterdam.
Yin, D. (1996) Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores. Free Rad. Biol. Med. 21, 871–888.
Brunk, U. T. and Sohal, R. S. (1991) Mechanisms of lipofuscin formation, in Membrane Lipid Oxidation vol II (Vigo-Pelfrey, C., ed), CRC Press, Boca Raton, FL, pp. 191–201
Brunk, U. T, Marzabadi, M. R. and Jones, C. B. (1992) Lipofuscin, lysosomes and iron, in Iron and Human Disease (Lauffer, R. B., ed), CRC Press, Boca Raton, FL. pp 237–260.
Brunk, U. T., Jones, C. B., and Sohal, R. S. (1992) A novel hypothesis of lipofuscinogenesis and cellular aging based on interactions between oxidative stress and autophagocytosis. Mut Res. 275, 395–403.
Sohal, R. S. and Brunk, U. T. (1990) Lipofuscin as an indicator of oxidative stress of aging, in Lipofuscin and Ceroid Pigments (Porta, E. ed.), Plenum, New York Adv Exp. Med. Biol Vol 226,. pp. 17–26.
Sohal, R. S. and Brunk, U. T. (1990) Mitochondrial production of proxidants and cellular senescence Mut Res. 275, 295–304
Marzabadi, M. R. Yin, D., and Brunk, U. T. (1992) Lipofuscin in a model system of cultured cardiac myocytes, in Free Radicals and Aging (Emerit, I. And Chance, B., eds.), Birkhauser Verlag, Basel Switzerland, pp 78–88.
Brunk, U. T. Wihlmark, U., Wrigstad, A., Roberg, K., and Nilsson, S-E. (1995) Accumulation of lipofuscin within retinal pigment epithelial cells results in enhanced sensitivity to photo-oxidation. Gerontology 41 (suppl 2), 201–212
Yin, D. and Nilsson, E. (1997) The preparation of artificial ceroid/lipofuscin by UV-oxidation of subcellular organelles. Mech. Ageing Dev 99, 61–78.
Brunk, U. T. and Ericsson, J. L. E. (1972) Electron microscopical studies on rat brain neurons: localization of acid phosphatase and mode of formation of lipofuscin bodies. J. Ultrastruct Res. 38, 1–15
Bjorkerud, S. (1964) Isolated lipofuscin granules—a survey of a new field Adv Gerontol Res 1, 257–288.
Siakotos, A. N. and Koppang, N. (1973) Procedures for the isolation of lipopigments from brain, heart and liver, and their properties: a review. Mech. Ageing Dev 2, 177–200
Elleder, M. (1981) Chemical characterization of age pigment, in Age Pigments (Sohal, R. S., ed), Elsevier, Amsterdam, pp 204–241
Patro, N., Patro, I. K., and Mathur, R. (1993) Changes in the properties of cardiac lipofuscin with age and environmental manipulation. Asian J. Exp. Sci. 7, 57–60.
Fletcher, B. L., Dillard, C. J., and Tappel, A. L. (1973) Measurement of fluorescent lipid peroxidation products in biological systems and tissues Anal. Biochem 52, 1–9
Desai, I. D., Fletcher, B. L., and Tappel, A. L (1975) Fluorescent pigments from uterus of vitamin E deficient rats. Lipids 10, 307–309
Csallany, A. S. and Ayaz, K. L. (1976) Quantitative determination of organic solvent soluble lipofuscin pigments in tissues. Lipids 11, 412–417.
Maeba, R., Shimasaki, H., Ueta, N., and Inoue, K. (1990) Accumulation of ceroid-like pigments in macrophages cultured with phosphatidylcholine liposomes in vitro Biochim. Biophys. Acta 1042, 287–293
Kikugawa, K., Kato, T., Yamaki, S., and Kasai, H. (1994) Examination of the extraction methods and re-evaluation of blue fluorescence generated in rat tissues in situ. Biol. Pharm Bull. 17, 9–15.
Ettershank, G., Macdonnell, I., and Croft, R. (1983) The accumulation of age pigment by the fleshfly sarcophage bullata parker (diptera: Sarcophagidae) Aust J. Zoology 31, 131–138.
Ettershank, G. (1984) A new approach to the assessment of longevity in the Antarctic krill euphausia superba. J. Crustac. Biol. 4, 295–305.
Tsuchida, M., Miura, T., and Aibara, K. (1987) Lipofuscin and lipofuscin-like substances Chem. Phys. Lipids 44, 297–325.
Porta, E. A., Mower, H. F., Moroye, M., Lee, C., and Palimbo, N. E. (1988) Differential features between lipofuscin (age pigment) and various experimentally produced ceroid pigments, in Lipofuscin-1987: State of the Art (Zs.-Nagy, I., ed.), Excerpta Medica, Amsterdam, pp. 341–374.
Eldred, G. E. and Katz, M. L. (1989) The autofluorescent products of lipid peroxidation may not be lipofuscin-like Free Rad. Biol. Med 7, 157–163
Yin, D. and Brunk, U. T. (1991) Microfluorometric and fluorometric lipofuscin spectral discrepancies A concentration dependent metachromatic effect? Mech. Ageing Dev. 59, 95–109.
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Yin, D., Brunk, U. (1998). Autofluorescent Ceroid/Lipofuscin. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:217
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DOI: https://doi.org/10.1385/0-89603-472-0:217
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