Abstract
A variety of oxidative stress-inducing agents, such as metals, ultra-violet (UV) light, heme, and hemoglobin have been implicated in the pathogenesis of the inflammatory process. The cellular response to such agents involves the production of a number of soluble mediators including acute phase proteins, eicosanoids, and various cytokines.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Shibahara, S, Muller, R. M, and Taguchi, H (1987) Transcriptional control of rat heme oxygenase by heat shock J Biol. Chem. 262, 12,889–12,892
Mitani, K, Fujita, H, Sassa, S., and Kappas, A (1989) Heat shock induction of heme oxygenase mRNA in human Hep3B hepatoma cells. Biochem Biophys Res Commun. 165, 437–441
Keyse, S M and Tyrrell, R. M. (1989) Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite Proc. Natl Acad Sci. USA 86, 99–103
Taketani, S, Kohno, H, Yoshinaga, T, and Tokunaga, R (1989) The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase. FEBS Lett 245, 173–176
Abraham, N G, Lin, J. H-C, Schwartzman, M L., Levere, R D, and Shibahara, S (1988) The physiological significance of heme oxygenase. Int. J Biochem. 20, 543–558
Stocker, R, Yamamoto, Y, McDonagh, A F, Glazer, A N, and Ames, B N (1987) Bilirubin is an antioxidant of possible physiological importance. Science 235, 1043–1046
Nath, K A, Balla, G, Vercellotti, G. M, Balla, J, Jacob, H. S., Levitt, M. D., and Rosenberg, M E. (1992) Induction of heme oxygenase is a rapid, protective response in rhabdomyolysis in the rat. J. Clin. Invest. 90, 267–270.
Paller, M S and Jacob, H S (1994) Cytochrome P-450 mediates tissue-damaging hydroxyl radical formation during reoxygenation of the kidney Proc. Natl Acad. Sci USA 91, 7002–7006
Stocker, R. (1990) Induction of haem oxygenase as a defence against oxidative stress. Free Rad Res Comm. 9, 101–112
McCoubrey, W K, Jr., Ewing, J. F., and Maines, M D (1992) Human heme oxygenase-2: characterization and expression of a full-length cDNA and evidence suggesting that the two HO-2 transcripts may differ by choice of a polyadenylation signal. Arch. Biochem. Biophys 295, 13–20
Shibahara, S, Yoshizawa, M, Suzuki, H, Takeda, K, Meguro, K, and Endo, K (1993) Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation J Biochem 113, 214–218
Cantoni, L., Rossi, C, Rizzardini, M., Gadina, M., and Ghezzi, P. (1991) Interleukin-1 and tumour necrosis factor induce hepatic haem oxygenase Biochem J. 279, 891–894.
Rizzardini, M, Terao, M, Falciani, F, and Cantoni, L (1993) Cytokine induction of haem oxygenase mRNA in mouse liver. Biochem. J. 290, 343–347.
Lutton, J. D, da Silva, J-L, Moquattash, S, Brown, A C, Levere, R D, and Abraham, N. G (1992) Differential induction of heme oxygenase in the hepatocarcinoma cell line (Hep3b) by environmental agents. J. Cell Biol. 49, 259–265.
Alam, J. and Zhining, D (1992) Distal AP-1 binding sites mediate basal level enhancement and TPA induction of the mouse heme oxygenase-1 gene. J. Biol Chem. 267, 21,894–21,900
Lavrovsky, Y., Schwartzman, M. L., Levere, R D, Kappas, A, and Abraham, N G. (1994) Identification of NFkB and AP-2 binding sites in the promoter region of the human heme oxygenase-1 gene. Proc. Natl. Acad. Sci USA 91, 5987–5991.
Becker-Andre, M. and Hahlbrock, K (1989) Absolute mRNA quantification using the polymerase chain reaction (PCR): a novel approach by a PCR aided transcript titration assay (PATTY) Nucleic Acids Res. 17, 9437–9446.
Wang, A M., Doyle, M V., and Mark, D.F. (1989) Quantitation of mRNA by the polymerase chain reaction Proc. Natl Acad. Sci. USA 86, 9717–9721.
Gilliland, G., Perrin, S, Blanchard, K., and Bunn, H. F. (1990) Analysis of cytokines mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc Natl. Acad. Sci USA 87, 2725–2729.
Cross, N. C. (1995) Quantitative PCR techniques and applications. Br J. Haematol. 89, 639–647
Murphy, L. D, Herzog, C. E., Rudick, J. B., Fojo, A. T, and Bates, S E (1990) Biochemistry 29, 10,351–10,356
Kutty, G., Hayden, B., Osawa, Y., Wiggert, B., Chader, G J, and Kutty R K. (1992) Heme oxygenase: expression in human retinal and modulation by stress agents in a human retinoblastoma cell model system. Curr. Eye Res. 11, 153–160
Yoshida, T., Biro, P, Cohen, T., Muller, R. M., and Shibahara, S. (1988) Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur J Biochem. 171, 457–461.
Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidium. Anal. Biochem. 162, 156–159.
Cross, N C, Feng, L, Chase, A., Bungey, J, Hughes, T P, and Goldman, J. M. (1993) Competitive polymerase chain reaction to estimate the number of BCR-ABL transcripts in chronic myeloid leukemia patients after bone marrow transplantation. Blood 82, 1929–1936.
Frenoy, N., Chabli, A, Sol, O, Goldschmit, E., Lemonnier, M. P., Misset, J L, and Debuire, B. (1994) Application of a new protocol for nested PCR to the detection of minimal residual bcr/abl transcripts. Leukemia 8, 1411–1414
Lion, T., Gaiger, A, Henn, T, Horth, E., Haas, O. A., Geissler K., and Gadner H (1995) Use of quantitative polymerase chain reaction to monitor residual disease in chronic myelogenous leukemia during treatment with interferon Leukemia 9, 1353–1360
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Abraham, N.G. (1998). Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:199
Download citation
DOI: https://doi.org/10.1385/0-89603-472-0:199
Publisher Name: Humana Press
Print ISBN: 978-0-89603-472-3
Online ISBN: 978-1-59259-254-8
eBook Packages: Springer Protocols