The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria

  • Stephen Gordon
  • Gavin Chung
  • Peter Andrew
Part of the Methods in Molecular Biology™ book series (MIMB, volume 101)

Abstracts

Bioluminescence is the product of two distinct enzymes, firefly luciferase and bacterial luciferase. The application of the firefly enzyme in the study of mycobacteria has been described by other groups (1,2) (see  Chapter 31). The bacterial-luciferase enzyme is a drmer of approx 80 kDa, consisting of an α- and β-subunit (3,4). It catalyzes the oxidation of long-chain fatty acids (>7 carbons) and reduced riboflavin, generating blue-green light (λ = 490 nm) in the process. The reaction can be written as follows: FMNH2 + RCHO + O2→ FMN + H2O + RCOOH + Light

Keywords

Agar DMSO Tuberculosis Aldehyde Barium 

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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Stephen Gordon
    • 1
  • Gavin Chung
    • 2
  • Peter Andrew
    • 3
  1. 1.Unité de Génétique Moléculaire BactérienneInstitut PasteurParisFrance
  2. 2.Chemotherapy DepartmentGlaxo-Wellcome Medical Research CentreStevenageUK
  3. 3.Department of Microbiology and ImmunologyUniversity of LeicesterLeicesterUK

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