Inhibitor-1, a Regulator of Protein Phosphatase 1 Function

  • John H. Connor
  • Hai Quan
  • Carey Oliver
  • Shirish Shenolikar
Part of the Methods in Molecular Biology™ book series (MIMB, volume 93)


With the discovery of numerous eukaryotic protein serine/threonine phosphatases, a classification scheme was developed that exploited some of the biochemical properties of these enzymes to expedite their identification (1). These properties included the preferential dephosphorylation of either α or β-subunits of phosphorylase kinase, the metal ion dependency displayed by some phosphatases for enzyme activity and their inhibition by two mammalian thermostable proteins, inhibitor-1 (I-1) and inhibitor-2 (I-2).


Okadaic Acid Apparent Molecular Weight Rabbit Skeletal Muscle Phosphorylase Kinase Coli BL21 
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Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • John H. Connor
    • 1
  • Hai Quan
    • 1
  • Carey Oliver
    • 1
  • Shirish Shenolikar
    • 1
  1. 1.Department of Pharmacology and Cancer BiologyDuke University Medical CenterDurham

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