Skip to main content
SpringerLink
Log in

Detection of Apoptotic or Necrotic Death in Neuronal Cells by Morphological, Biochemical, and Molecular Analysis

  • Protocol
Apoptosis Techniques and Protocols

Part of the book series: Neuromethods ((NM,volume 29))

Abstract

During embryogenesis of vertebrates, programmed cell death (or apoptosis) is an important event involved in tissue formation and organogenesis (Ernst, 1926; Glücksmann, 1951; Kerr et al., 1972). In the nervous system, programmed cell death of sensory and motor neurons occurs in embryonic stages, but also maturation of central and peripheral nervous system is accompanied by extensive deletion of supernumerary neurons. Furthermore, neuronal cell death is not only part of normal brain development, it also occurs in neurodegenerative disorders.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Arispe N., Pollard H and Rojas E. (1993) Grant multilevel catron channels formed by Alzhelmer disease amylold beta-protein [A beta P-(1-40)] in bllayer membranes Proc Natl Acad Sci USA 90, 10,573–10,577.

    Article  PubMed  CAS  Google Scholar 

  • Batistatou A and Greene L. A. (1991) Aurmtricarboxyhc acid rescues PC12 cells and sympathetrc neurons from cell death caused by nerve growth factor deprivatron correlatron with suppression of endonuclease activrty J Cell Biol 115, 461–471.

    Article  PubMed  CAS  Google Scholar 

  • Behl C., Davis J. B., Klier F. G, and Schubert D (1994) Amylold B peptide mduces necrosis rather than apoptosrs. Brain Res 645, 253–264.

    Article  PubMed  CAS  Google Scholar 

  • Bendotti C., Forloni G. L., Morgan R. A., O’Hara B. F., Oster-Granite M. L., Reeves R. H, Gearhart J D, and Coyle J T (1988) Neuroanatomrcal localizatron and quantrfication of amyloid precursor protein mRNA by in situ hybridization in the brains of normal, aneuploid, and lesloned mice. Proc Natl Acad Sci USA 85, 3628–3632.

    Article  PubMed  CAS  Google Scholar 

  • Cai X D, Golde T. E., and Younkin S G. (1993) Release of excess amyloid beta protein from a mutant amyloid β protein precursor. Science 259, 514–516

    Article  PubMed  CAS  Google Scholar 

  • Chartier-Harlin M. C, Crawford F, Houlden H., Warren A, Hughes D, Fidani L., Goate A, Rossor M., Roques P., Hardy J., and Mullan M (1991) Early-onset Alzheimer’s disease caused by mutations at codon 717 of the beta-amylord precursor protein gene. Nature 353, 844–846

    Article  PubMed  CAS  Google Scholar 

  • Citron M., Oltersdorf T, Haass C., McConlogue L., Hung A. Y., Seubert P, Vigo-Pelfrey C, Lieberburg I, and Selkoe D. J. (1992) Mutation of the beta-amyloid precursor protein in familial Alzheimer’s disease increases beta-protem production Nature 360, 672–674

    Article  PubMed  CAS  Google Scholar 

  • Cohen J J and Duke R C (1984) Glucocorticold activation of a calciumdependent endonuclease in thymocyte nuclei leads to cell death. J Immunol 132, 38–42

    PubMed  CAS  Google Scholar 

  • Emre M., Geula C, Ransrl B J, and Mesulam M. M (1992) The acute neurotoxicrty and effects upon cholmergrc axons of mtracerebrally injected beta-amyloid in the rat brain Neurobiol Aging 13, 553–559

    Article  PubMed  CAS  Google Scholar 

  • Ernst M (1926) Uber Untergang von Zellen wahrend der normalen Entwlcklung bei Wrrbeltreren. Z Anat Entwzcklungsgesch 79, 228–262.

    Article  Google Scholar 

  • Forlorn G, Chiesa R., Smiroldo S, Verga L, Salmona M, Tagliavini F, and Angerettl N (1993) Apoptosis mediated neurotoxlcity induced by chronic apphcatron of beta amyloid fragment 25–35. Neuroreport 4, 523–526.

    Article  Google Scholar 

  • Games D., Khan K M, Soriano F G., Keim P S., Davis D. L, Bryant K, and Lieberburg I (1992) Lack of Alzheimer pathology after betaamyloid protein mlections in rat bram Neurobiol Aging 13, 569–576.

    Article  PubMed  CAS  Google Scholar 

  • Glenner G. G and Wong C W (1984) Alzheimer’s disease: initial report of the purification and characterization of a novel cerebrovascular amyloid protein Biochem Biophys Res Commun 120, 885–890.

    Article  PubMed  CAS  Google Scholar 

  • Glucksmann A. (1951) Cell death in normal vertebrate ontogeny. Biol Rev 26, 59–86.

    Article  Google Scholar 

  • Goate A, Chartier-Harlin M. C, Mullan M, Brown J, Crawford F., Fidani L., Gmffra L, Haynes A., Irving N, James L, Mant R., Newton P, Rooke K., Roques P., Talbot C., Perrcak-Vance M., Roses A, Williamson R., Rossor M, Owen M., and Hardy J (1991) Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer’s disease. Nature 349, 704–706

    Article  PubMed  CAS  Google Scholar 

  • Goldgaber D, Lerman M. I., McBride O. W, Saffiotti U., and Gajdusek D. C (1987) Characterization and chromosomal localization of a cDNA encoding brain amyloid of Alzheimer’s disease. Science 235, 877–880.

    Article  PubMed  CAS  Google Scholar 

  • Gschwind M and Huber G. (1995) Apoptotic cell death induced by β-amyloid (l–42) peptide is cell type dependent. J Neurochem 65, 292–300.

    Article  PubMed  CAS  Google Scholar 

  • Hansen M B, Nielsen S. E., and Berg K. (1989) Re-exammation and further development of a precise and rapid dye method for measurmg cell growth/cell kill J Immunol Methods 119, 203–210.

    Article  PubMed  CAS  Google Scholar 

  • Johnston J. A, Cowburn R. F., Norgren S, Wiehager B, Vemzelos N., Wmbald B., Vigo-Pelfrey C., Schenk D, Lannfelt L., and O’Neill C (1994) Increased β-amyloid release and levels of amyloid precursor protein (APP) in fibroblast cell lmes from family members with the Swedish Alzheuner’s disease APP670/671 mutation FEBS Lett 354, 274–278

    Article  PubMed  CAS  Google Scholar 

  • Kang J., Lemaire H. G, Unterbeck A., Salbaum J. M., Masters C L., Grzeschik K H, Multhaup G, Beyreuther K, and Muller-Hill B (1987) The precursor of Alzheimer’s disease amyloid A4 protein resembles a cell-surface receptor Nature 325, 733–736

    Article  PubMed  CAS  Google Scholar 

  • Katzman R and Saitoh T (1991) Advances in Alzheimer’s disease FASEBJ 5, 278–286.

    CAS  Google Scholar 

  • Kerr J. F R, Wyllie A H., and Currie A R. (1972) Apoptosis: a basic biological phenomenon with wideranging implications in tissue kinetics. Br J Cancer 26, 239–257

    Article  PubMed  CAS  Google Scholar 

  • Kowall N W, McKee A. C, Yankner B. A, and Beal M. F (1992) In vivo neurotoxicity of beta-amyloid [beta(l–40)] and the beta(25–35) fragment. Neurobiol. Aging 13, 537–542.

    Article  PubMed  CAS  Google Scholar 

  • Loo D T., Copani A, Pike C J, Whittemore E R, Walencewicz A J, and Cotman W. (1993) Apoptosis is induced by beta-amyloid in cultured central nervous system neurons Proc Natl Acad Sci USA 90, 7951–7955

    Article  PubMed  CAS  Google Scholar 

  • Mullan M., Crawford F, Axelman K, Houlden H., Lilius L, Winbald B, and Lannfelt L. (1992) A pathogenic mutation for probable Alzheimer’s disease in the APP gene at the N-terminus of β-amyloid Nat Genet 1, 345–347.

    Article  PubMed  CAS  Google Scholar 

  • Murrell J, Farlow M., Ghetti B, and Benson M. D (1991) A mutation in the amyloid precursor protein associated with hereditary Alzheimer’s disease. Science 254, 97–99

    Article  PubMed  CAS  Google Scholar 

  • Selkoe D. J. (1991) The molecular pathology of Alzheimer’s disease. Neuran 6, 487–498

    Article  CAS  Google Scholar 

  • Stephenson D T and Clemens J A. (1992) In VIVO effects of beta-amyloid implants in rodents: lack of potentiation of damage associated with transient global forebrain ischemia Brm Res 586, 235–246

    Article  CAS  Google Scholar 

  • Suzuki N., Cheung T T., Cal X D., Odaka A., Otvos L, Eckman C., Golde T E., and Younkin S G. (1994) An increased percentage of long amyloid beta protein secreted by familial amyloid beta protein precursor (beta APP717) mutants Science 264, 1336–1340.

    Article  PubMed  CAS  Google Scholar 

  • Tamaoka A., Odaka A., Ishibashi Y, Usami M., Sahara N., Suzuki N., Nukina N, Mizusawa H, Shoji S, Kanazawa I., and Mori H (1994) APP717 Missense mutation affects the ratio of amyloid β protein species (Aβ1-42/43 and Aβ1-40) in familial Alzheimer’s disease brain J Biol Chem 269, 32,721–32,724.

    PubMed  CAS  Google Scholar 

  • Tanzi R E, Gusella J F, Watkins P C, Bruns G. A, St George-Hyslop P H, Van Keuren M L, Patterson D., Pagan S, Kurnit D M., and Neve R L (1987) Amyloid β protein gene. cDNA, mRNA distrrbutron, and genetic linkage near the Alzheimer locus. Science 235, 880–884.

    Article  PubMed  CAS  Google Scholar 

  • Tanzi R. E, St George-Hyslop P H., and Gusella J F (1989) Molecular genetic approaches to Alzheimer’s disease. Trends Neurosci 12, 152–158

    Article  PubMed  CAS  Google Scholar 

  • Wyllie A. H., Kerr J F. R, and Currle A R (1980) Cell death the significance of apoptosls Int Rev Cytol 68, 251–306

    Article  PubMed  CAS  Google Scholar 

  • Wylhe A. H, Morris R G, Smith A L, and Dunlop D (1984) Chromatin cleavage in apoptosis association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142, 67–77

    Article  Google Scholar 

  • Yankner B A, Dawes L. R, Fisher S, Villa-Komaroff L., Oster-Granite M L., and Neve R L. (1989) Neurotoxiclty of a fragment of the amyloid precursor associated with Alzhelmer’s disease Science 245, 417–420.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pharma Division, Preclinical CNS Research, F. Hoffmann La-Roche Ltd., Basel, Switzerland

    Martin Gschwind & Gerda Huber

Authors
  1. Martin Gschwind
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gerda Huber
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Douglas Hospital Research Centre, Verdun, Canada

    Judes Poirier

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Humana Press Inc.

About this protocol

Cite this protocol

Gschwind, M., Huber, G. (1997). Detection of Apoptotic or Necrotic Death in Neuronal Cells by Morphological, Biochemical, and Molecular Analysis. In: Poirier, J. (eds) Apoptosis Techniques and Protocols. Neuromethods, vol 29. Humana Press. https://doi.org/10.1385/0-89603-451-8:13

Download citation

  • DOI: https://doi.org/10.1385/0-89603-451-8:13

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-451-8

  • Online ISBN: 978-1-59259-634-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation