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Transcriptional Footprinting of Drug–DNA Interactions

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Drug-DNA Interaction Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 90))

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Abstract

There are now approx 50 registered anticancer drugs that are in routine clinical use as chemotherapeutic agents (1–3). Of these, over half are known to interact with DNA, either by intercalation (e.g., doxorubicin, mitoxantrone), groove binding (e.g., distamycin), formation of adducts or crosslinks (e.g., melphalan, cisplatin, mitomycin C), or by incorporation of modified bases (e.g., 5-fluorouracil, 6-thioguanine). There has therefore been a great effort over many years to establish where on the DNA these drugs interact, with the expectation that a good understanding of the nature of the DNA receptor site would lead to the design of a new generation of these drugs.

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Authors and Affiliations

  1. School of Biochemistry, La Trobe University, Bundoora, Victoria, Australia

    Don R. Phillips & Carleen Cullinane

Authors
  1. Don R. Phillips
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  2. Carleen Cullinane
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Editor information

Editors and Affiliations

  1. University of Southampton, UK

    Keith R. Fox

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© 1997 Humana Press Inc., Totowa, NJ

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Phillips, D.R., Cullinane, C. (1997). Transcriptional Footprinting of Drug–DNA Interactions. In: Fox, K.R. (eds) Drug-DNA Interaction Protocols. Methods in Molecular Biology™, vol 90. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-447-X:127

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  • DOI: https://doi.org/10.1385/0-89603-447-X:127

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-447-1

  • Online ISBN: 978-1-59259-574-7

  • eBook Packages: Springer Protocols

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