Abstract
Since its discovery by Prof. Sir Alec Jeffreys FRS in 1984, DNA fingerprinting using multilocus VNTR probes (MLPs) has been widely used in the field of human identification (1,2). Although multilocus probes were used in forensic investigations in the late 1980s (3), forensic samples often yield too little undegraded DNA for the production of a complete DNA fingerprint. Therefore, single-locus VNTR probes (SLPs) are now more widely used in crime-stain analysis. The major application of MLPs is in the analysis of relationships, DNA fingerprinting remaining the most accurate method available for paternity testing (4,5). Because MLPs detect multiple hypervariable loci simultaneously, the technology is especially powerful in the analysis of complex relationships and cases of incest. DNA fingerprinting is also widely used in establishing zygosity in twins and in relationship-testing in animals (6,7).
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References
Jeffreys, A. J., Wilson, V., and Thein, S. L. (1985) Hypervariable “minisatellite” regions in human DNA. Nature 314, 67–73.
Jeffreys, A. J., Brookfield, J. F. Y., and Semeonoff, R. (1985) Positive identification of an immigration test-case using human DNA fingerprints. Nature 317, 818,819.
Gill, P., Jeffreys, A. J., and Werrett, D. J. (1985) Forensic application of DNA “fingerprints”. Nature 318, 577–579.
Smith, J. C., Newton, C. R., Alves, A., Anwar, R., Jenner, D., and Markham, A. F. (1990) Highly polymorphic minisatellite DNA probes. Further evaluation for individual identification and paternity testing. J. Forensic Sci. Soc. 30, 3–18.
Pena, S. D. J. and Chakraborty, R. (1994) Paternity testing in the DNA era. Trends Genet. 10, 204–209.
Hill, A. V. S. and Jeffreys, A. J. (1985) Use of minisatellite DNA probes for determination of twin zygosity at birth. Lancet ii, 1394,1395.
Morton, D. B., Yaxley, R. E., Patel, I., Jeffreys, A. J., Howes, S. J., and Debenham, P. G. (1987) Use of DNA fingerprint analysis in identification of the sire. Veteri-nary Rec. 121, 592–594.
Jeffreys, A. J., Wilson, V., Thein, S. L., Weatherall, D. J., and Ponder, B. A. J. (1986) DNA “fingerprints” and segregation analysis of multiple markers in human pedigrees. Am. J. Hum. Genet. 39, 11–24.
Jeffreys, A. J., Turner, M., and Debenham, P. G. (1991) The efficiency of multilocus DNA fingerprint probes for individualisation and establishment of family relationships determined from extensive casework. Am. J. Hum. Genet. 48, 824–840.
Bruford, M. W., Hanotte, O., Brookfield, J. F. Y., and Burke, T. (1992) Single locus and multilocus DNA fingerprinting, in Molecular Genetic Analysis of Populations (Hoelzel, A. R., ed.), IRL, Oxford, UK, pp. 225–269.
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© 1998 Humana Press Inc., Totowa, NJ
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Rysiecki, G., Brookfield, J.F.Y. (1998). Interpretation and Statistical Evaluation of Multilocus DNA Fingerprints in Paternity and Relationship Testing. In: Lincoln, P.J., Thomson, J. (eds) Forensic DNA Profiling Protocols. Methods in Molecular Biology, vol 98. Humana Press. https://doi.org/10.1385/0-89603-443-7:97
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DOI: https://doi.org/10.1385/0-89603-443-7:97
Publisher Name: Humana Press
Print ISBN: 978-0-89603-443-3
Online ISBN: 978-1-59259-204-3
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