Abstract
Quantification of template DNA is an essential step in the analysis of samples using polymerase chain reaction (PCR). Once aPCR reaction has been optimized, the amplification of too little genomic DNA may yield only partial results, and the addition of too much template may increase the tendency for amplification of artifact products.
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References
Walsh, P. S., Metzger, D. A., and Higuchi, R. (1991) Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 4, 506–513.
Walsh, P. S., Varlaro, J., and Reynolds, R. (1992) A rapid chemiluminescent method for quantitation of human DNA. Nucleic Acids Res. 20, 5061–5065.
Waye, J. S. and Willard, H. F. (1986) Structure, organisation and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome. Molec. Cell. Biol. 6, 3156–3165.
Whitehead, T. P., Thorpe, G. H. G., Carter, T. J. N., Groucutt, C., and Kricka, L. J. (1983) Enhanced luminescence procedure for sensitive determination of per-oxidase-labelled conjugates in immunoassay. Nature 305, 158,159.
Velleman, S. G. (1995) Quantifying immunoblots with a digital scanner. Biotechniques 18, 1056–1058.
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© 1998 Humana Press Inc., Totowa, NJ
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Andersen, J. (1998). Quantification of DNA By Slot-Blot Analysis. In: Lincoln, P.J., Thomson, J. (eds) Forensic DNA Profiling Protocols. Methods in Molecular Biology, vol 98. Humana Press. https://doi.org/10.1385/0-89603-443-7:33
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DOI: https://doi.org/10.1385/0-89603-443-7:33
Publisher Name: Humana Press
Print ISBN: 978-0-89603-443-3
Online ISBN: 978-1-59259-204-3
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