Skip to main content

Quantification of DNA By Slot-Blot Analysis

  • Protocol
Forensic DNA Profiling Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 98))

  • 1933 Accesses

Abstract

Quantification of template DNA is an essential step in the analysis of samples using polymerase chain reaction (PCR). Once aPCR reaction has been optimized, the amplification of too little genomic DNA may yield only partial results, and the addition of too much template may increase the tendency for amplification of artifact products.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Walsh, P. S., Metzger, D. A., and Higuchi, R. (1991) Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 4, 506–513.

    Google Scholar 

  2. Walsh, P. S., Varlaro, J., and Reynolds, R. (1992) A rapid chemiluminescent method for quantitation of human DNA. Nucleic Acids Res. 20, 5061–5065.

    Article  PubMed  CAS  Google Scholar 

  3. Waye, J. S. and Willard, H. F. (1986) Structure, organisation and sequence of alpha satellite DNA from human chromosome 17: evidence for evolution by unequal crossing-over and an ancestral pentamer repeat shared with the human X chromosome. Molec. Cell. Biol. 6, 3156–3165.

    PubMed  CAS  Google Scholar 

  4. Whitehead, T. P., Thorpe, G. H. G., Carter, T. J. N., Groucutt, C., and Kricka, L. J. (1983) Enhanced luminescence procedure for sensitive determination of per-oxidase-labelled conjugates in immunoassay. Nature 305, 158,159.

    Article  Google Scholar 

  5. Velleman, S. G. (1995) Quantifying immunoblots with a digital scanner. Biotechniques 18, 1056–1058.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Andersen, J. (1998). Quantification of DNA By Slot-Blot Analysis. In: Lincoln, P.J., Thomson, J. (eds) Forensic DNA Profiling Protocols. Methods in Molecular Biology, vol 98. Humana Press. https://doi.org/10.1385/0-89603-443-7:33

Download citation

  • DOI: https://doi.org/10.1385/0-89603-443-7:33

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-443-3

  • Online ISBN: 978-1-59259-204-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics