Abstract
The polymerase chain reaction (PCR) has offered the forensic scientist a new range of sensitivity in the examination of forensic samples. PCR has been successfully used to amplify specific DNA fragments from extremely small amounts of DNA present on cigaret butts (1,2), postage stamps (3), envelope sealing flaps, and other saliva-stained materials (4). In addition to DNA typing results, it is at times desirable to confirm the presence of saliva by the simultaneous employment of an amylase assay. This chapter describes methods that are routinely and successfully used on forensic casework samples.
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References
Hochmeister, M., Budowle, B., Borer, U. V., Comey, C. T., and Dirnhofer, R. (1991) PCR-based typing of DNA extracted from cigarette butts. Int. J. Legal Med. 104, 229–233.
Schmitter, H. and Sonntag, M. L. (1995) STR-analysis on cigarette butts. Experiences with casework material. Klin. Lab. 41, 177–180.
Hopkins, B., Williams, N. J., Webb, M. B. T., Debenham, P. G., and Jeffreys, A. J. (1994) The use of minisatellite variant repeat-polymerase chain reaction (MVR-PCR) to determine the source of saliva on a used postage stamp. J. Forensic Sci. 39, 526–532.
Walsh, D. J., Corey, A. C., Cotton, R. W., Forman, L., Herrin, G. L., Word, C. J., and Garner, D. D. (1992) Isolation of deoxyribonucleic acid (DNA) from saliva and forensic science samples containing saliva. J. Forensic Sci. 37, 387–395.
Budowle, B. and Baechtel, F. S. (1990) Modifications to improve the effectiveness of restriction fragment length polymorphism typing. Appl. Theoret. Electro-phoresis 1, 181–187.
Budowle, B., Chakraborty, R., Giusti, A. W., Eisenberg, A. J., and Allen, R. C. (1991) Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE. Am. J. Hum. Genet. 48, 137–144.
Lee, H. C. and Gaensslen, R. E. (eds.) (1991) Advances in Fingerprint Technology. Elsevier, New York.
Singer-Sam, J., Tanguay, R. L., and Riggs, A. D. (1989) Use of Chelex to improve the PCR signal from a small number of cells. Amplifications 3, 11.
Hagelberg, E., Sykes, B., and Hedge, R. (1989) Ancient bone DNA amplified. Nature 342, 485,486.
Hagelberg, E., Gray, I. C., and Jeffreys, A. J. (1991) Identification of the skeletal remains of a murder victim by DNA analysis. Nature 352, 427–429.
Hochmeister, M., Budowle, B., Borer, U. V., Eggmann, U., Comey, C. T., and Dirnhofer, R. (1991) Typing of deoxyribonucleic acid (DNA) extracted from compact bone from human remains. J. Forensic Sci. 36, 1649–1661.
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© 1998 Humana Press Inc., Totowa, NJ
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Hochmeister, M.N., Rudin, O., Ambach, E. (1998). PCR Analysis from Cigaret Butts, Postage Stamps, Envelope Sealing Flaps, and Other Saliva-Stained Material. In: Lincoln, P.J., Thomson, J. (eds) Forensic DNA Profiling Protocols. Methods in Molecular Biology, vol 98. Humana Press. https://doi.org/10.1385/0-89603-443-7:27
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DOI: https://doi.org/10.1385/0-89603-443-7:27
Publisher Name: Humana Press
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