Abstract
Fluorescent in situ hybridization (FISH) is a sensitive and powerful method for mapping and positioning DNA sequences in mammalian and other genome systems (1–3). DNA sequences ranging in size from <1 kb to several megabases can be localized to a specific chromosome site. The DNA is first labeled by nick translation with a non-radioactive immunofluorescent compound such as biotin-11-dUTP or digoxigenin-11-dUTP, then hybridized overnight to cell or chromosome preparations. The resulting signal can be detected under ultraviolet light with filters of wavelengths specific to the fluorescent compound.
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© 1997 Humana Press Inc., Totowa, NJ
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Cannizzaro, L.A., Shi, G. (1997). Fluorescent In Situ Hybridization (FISH) for DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle. In: Pollard, J.W., Walker, J.M. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-441-0:313
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DOI: https://doi.org/10.1385/0-89603-441-0:313
Publisher Name: Humana Press, Totowa, NJ
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