Abstract
The maintenance of normal primitive hemopoietic cell types in culture for long periods can be achieved either by culture of hemopoietic cells in association with bone marrow-derived stromal cells, as in long-term cultures, or by establishing factor-dependent (FDCP-Mix) primitive hemopoietic cell lines. The long-term culture technique and a reproducible method for establishing FDCP-Mix cell lines are described here (1,2).
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References
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© 1997 Humana Press Inc., Totowa, NJ
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Spooncer, E., Dexter, T.M. (1997). Culturing Primitive Hemopoietic Cells. In: Pollard, J.W., Walker, J.M. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-441-0:209
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DOI: https://doi.org/10.1385/0-89603-441-0:209
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-441-9
Online ISBN: 978-1-59259-561-7
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