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Basic Cell Culture Protocols pp 185–207Cite as

Culture of Cells From Human Tumors of the Nervous System on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 75))

Abstract

Cell culture has become an integral part of the daily routine of most biological laboratories. Many genetic tests, chemo-resistance testing for chemotherapy, and the study of deregulated cell proliferation in cancer, cannot be performed without cultured cells. In endocrinology and developmental biology, elaborate cell culture techniques allow the investigation of regulatory processes and cellular interactions in in vitro systems, in which the experimental conditions can be defined as in no other experimental paradigm.

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References

  1. Bottenstein, J. E. and Sato, G. H. (1980) Fibronectin and polylysine requirement for proliferation of neuroblastoma cells in defined medium. Exp. Cell Res. 129, 361–366.

    Article  CAS  PubMed  Google Scholar 

  2. Terranova, V. P., Aumalley, M., Sultan, L. H., Martin, G. R., and Kleinman, H. K. (1986) Regulation of cell attachment and cell number by fibronectin and laminin. J. Cell. Physiol. 127, 473–419.

    Article  CAS  PubMed  Google Scholar 

  3. Couchmann, J. R., Hook, M., Rees, D., and Timpl, R. (1983) Adhesion, growth and matrix production by fibroblasts on laminin substrates. J. Cell. Biol. 96, 177–183.

    Article  Google Scholar 

  4. Varani, J., Carey, T. E., Fligiel, S. E. G., McKeever, P. E., and Dixit, V. (1987) Tumor type-specific differences in cell-substrate adhesion among human tumor cell lines. Int. J. Cancer 39, 397–403.

    Article  CAS  PubMed  Google Scholar 

  5. Gospodarowicz, D., Vlodavsky, I., and Savion, N. (1981) The role of fibroblast growth factor and the extracellular matrix in the control of proliferation and differentiation of corneal endothelial cells. Vison Res. 21, 87–103.

    Article  CAS  Google Scholar 

  6. Gospodarowicz, D., Cohen, D., and Fujii, D. K. (1982) Regulation of cell growth by the basal lamina and plasma factors: relevance to embryonic control of cell proliferation and differentiation, in Cold Spring Harbor Conference on Cell Proliferation, vol. 9, Cold Spring Harbor Laboratories, Cold Spring Harbor, NY, pp. 95–124.

    Google Scholar 

  7. Lichtner, R. B., Belloni, P. N., and Nicolson G. L. (1989) Differential adhesion of metastatic rat mammary carcinoma cells to organ-derived microvessel endothelial cells and subendothelial matrix. Exp. Cell Biol. 57, 146–152.

    CAS  PubMed  Google Scholar 

  8. Pavelic, K., Bulbul, M. A., Slocum, H. K., Pavelic, Z. P., Rustum, Y. M., Niedbala, M. J., and Bernacki, R. J. (1986) Growth of human urological tumors on extracellular matrix as a model for the in vitro cultivation of primary tumor explants. Cancer Res. 46, 3653–3662.

    CAS  PubMed  Google Scholar 

  9. Cheitlin, R., Westphal, M., Cabrera C. M., Fujii, D. K., Snyder, J., and Fitzgerald, P. A. (1988) Cushing’s syndrome due to bilateral adrenal macronodular hyperplasia with undetectable ACTH: cell culture of adenoma cells on extracellular matrix. Hormone Res. 29, 162–167.

    Article  CAS  PubMed  Google Scholar 

  10. Westphal, M., Jaquet, P., and Wilson, C. B. (1986) Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions. Acta Neuropath. 71, 142–149.

    Article  CAS  PubMed  Google Scholar 

  11. Westphal, M., Hahn, H., and Ludecke, D. K. (1987) Culture of dispersed cells from human pituitry adenomas from acromegalic patients on extracellular matrix, in Growth Hormone, Growth Factors and Akromegaly (Ludecke, D. K. and Tolis, G., eds.), Raven, New York, pp. 125–133.

    Google Scholar 

  12. Westphal, M., Hansel, M., Brunken, M., Konig, A., Koppen, J. A., and Herrman, H. D. (1987) Initiation of primary cell cultures from human intracranial tumors on extracellular matrix from bovine corneal endothelial cells. Exp. Cell. Biol. 55, 152–163.

    CAS  PubMed  Google Scholar 

  13. Hynes, R. O. (1992) Integrins: versatility, modulation and signalling in cell adhesion. Cell 69, 11–25.

    Article  CAS  PubMed  Google Scholar 

  14. Alanko, T., Tienari, J., Lehtonen E., and Saksela, O. (1994) Development of FGF-dependency in human embryonic carcinoma cells after retinoic acid-induced differentiation. Dev. Biol. 161, 141–153.

    Article  PubMed  Google Scholar 

  15. Adams, J. C. and Watt, F. M. (1993) Regulation of development and differentiation by extracellular matrix. Development 117, 1183–1198.

    CAS  PubMed  Google Scholar 

  16. Vlodavsky, I., Lui, G. M., and Gospodarowicz, D. J. (1980) Morphological apearance, growth behavior and migratory activity of human tumor cells maintained on extracellular matrix versus plastic. Cell 19, 607–616.

    Article  CAS  PubMed  Google Scholar 

  17. Payne, H. R. and Lemmon, V. (1993) Glial cells of the O-2A lineage bind preferentially to N-cadherin and develop distinct morphologies. Dev. Biol. 159, 595–607.

    Article  CAS  PubMed  Google Scholar 

  18. Rutka, J. T. (1986) Effects of extracellular matrix proteins on the growth and differentiation of an anaplastic glioma cell line. Can. J. Neurol. Sci. 13, 301–30x.

    CAS  PubMed  Google Scholar 

  19. Westphal, M., Hansel, M., Nausch, H., Rohde, E., and Herrmann, H. D. (1990) Culture of human brain tumors on an extracellular matrix derived from bovine corneal endothelial cells and cultured human glioma cells, in Methods in Molecular Biology, vol. 5: Animal Cell Culture (Pollard, J. W. and Walker, J. M., eds.), Humana Press, Clifton, NJ, pp. 113–131.

    Google Scholar 

  20. Weiner, R. I., Bethea, C. L., Jaquet, P., Ramsdell, J. S., and Gospodarowicz, D. J. (1983) Culture of dispersed anterior pituitary cells on extracellular matrix. Methods Enzymol. 103, 287–294.

    Article  CAS  PubMed  Google Scholar 

  21. Westphal, M., Hansel, M., Hamel, W., Kunzmann, R., and Holzel, F. (1994) Karyotype analysis of 20 human glioma cell lines. Acta. Neurochir. 126, 17–26.

    Article  CAS  PubMed  Google Scholar 

  22. Cardwell, M. C. and Rome, L. H. (1988) Evidence that an RGD-dependent receptor mediates the binding of oligodendrocytes to a novel ligand in a glial-derived matrix. J. Cell Biol. 107, 1541–1549.

    Article  CAS  PubMed  Google Scholar 

  23. Gospodarowicz, D., Cheng, J., Lut, G. M., Baird, A., and Bohlen, P. (1984) Isolation of brain fibroblast growth factor by heparin sepharose affinity chromatography: identity with pituitary fibroblast growth factor. Proc. Natl. Acad. Sci. USA 81, 6963–6967.

    Article  CAS  PubMed  Google Scholar 

  24. Kleihues, P., Burger, P. C., and Scheithauer, B. W. (1993) The new WHO classification of brain tumors. Brain Pathol. 3, 255–268.

    Article  CAS  PubMed  Google Scholar 

  25. Liotta, L. A. and Stetler-Stevenson, W. G. (1991) Tumor invasion and metastasis, an imbalance of positive and negative regulation. Cancer Res. 51, 5054–5059.

    Google Scholar 

  26. Shapiro, J. R. (1986) Biology of gliomas: chromosomes, growth factors and oncogenes. Semin. Oncol. 13, 4–15.

    CAS  PubMed  Google Scholar 

  27. Giese, A., Rief, M., and Berens, M. (1994) Determinants of human glioma cell migration. Cancer Res. 54, 3887–3904.

    Google Scholar 

  28. Giese, A., Loo, M. A., Reef, M. D., Tran, N., and Berens, M. E. (1995) Substrates for astrocytoma invasion. Neurosurgery, 37, 294–302.

    Article  CAS  PubMed  Google Scholar 

  29. Mucke, L. and Rockenstein, E. M. (1993) Prolonged delivery of transgene products to specific brain regions by migratory astrocyte grafts. Transgene 1, 3–9.

    Google Scholar 

  30. Westphal, M., Stavrou, D., Nausch, H., Valdueza, J. M., and Herrmann, H.-D. (1994) Human neurocytoma cells in culture show characteristics of astroglial differentiation. J. Neurosci. Res. 38, 698–704.

    Article  CAS  PubMed  Google Scholar 

  31. Westphal, M., Meissner, H., and Herrmann, H. D. (1996) Astrocytic differentiation of human neurocytoma cells is a default pathway. J. Neurocytol., submitted.

    Google Scholar 

  32. Vlodavsky, I., Folkman, J., Sullivan, R., Fridman, R., Ishai-Michaeli, R., Sasse, J., and Klagsbrun, M. (1987) Endothelial cell-derived basic fibroblast growth factor, synthesis and deposition into subendothelial extracellular matrix. Proc. Natl. Acad. Sci. USA 84, 2292–2296.

    Article  CAS  PubMed  Google Scholar 

  33. Aviezer, D., Hecht, D., Safran, M., Eisinger, M., David, G., and Yayon, A. (1994) Perlecan, basal lamina proteoglycan, promotes basic fibroblasrt growth factor receptor binding, mitogenesis and angiogenesis. Cell 79, 1005–1013.

    Article  CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Neurosurgery, University Hospital Hamburg Eppendorf, Hamburg, Germany

    Manfred Westphal, Alf Giese, Hildegard Meissner & Dorothea Zirkel

Authors
  1. Manfred Westphal
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  2. Alf Giese
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  3. Hildegard Meissner
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  4. Dorothea Zirkel
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Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, NY

    Jeffrey W. Pollard

  2. University of Hertfordshire, Hatfield, Herts, UK

    John M. Walker

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© 1997 Humana Press Inc., Totowa, NJ

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Westphal, M., Giese, A., Meissner, H., Zirkel, D. (1997). Culture of Cells From Human Tumors of the Nervous System on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells. In: Pollard, J.W., Walker, J.M. (eds) Basic Cell Culture Protocols. Methods in Molecular Biology™, vol 75. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-441-0:185

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  • DOI: https://doi.org/10.1385/0-89603-441-0:185

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-441-9

  • Online ISBN: 978-1-59259-561-7

  • eBook Packages: Springer Protocols

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