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Visualization of Cell Replication Using Antibody to Proliferating Cell Nuclear Antigen

  • James Hyde-Dunn
  • Gareth E. Jones
Part of the Methods in Molecular Biology™ book series (MIMB, volume 75)

Abstract

The proliferating cell nuclear antigen (PCNA) is the 37-kDa molecular weight auxiliary protein of DNA polymerase δ (1). PCNA is a very conserved and highly regulated protein necessary for cell cycle progression. PCNA is associated with cyclin A in both soluble and insoluble fractions, whereas it is associated with cyclin D1 only in the soluble fraction (2). PCNA translocates from the cytoplasm to the nucleus at the beginning of S-phase, is required for entry into S-phase, and forms multiple high molecular weight complexes with cdk2 at late G1 or early S-phase (2). This cell-cycle distribution of PCNA may be employed as a marker of DNA replication.

Keywords

Proliferate Cell Nuclear Antigen Gentle Agitation Cell Side Bovine Serum Albumin Concentration Nonspecific Background Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1997

Authors and Affiliations

  • James Hyde-Dunn
    • 1
  • Gareth E. Jones
    • 1
  1. 1.Department of Anatomy, The Randall InstituteKing’s College LondonUK

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