Visualization of Cell Replication Using Antibody to Proliferating Cell Nuclear Antigen

  • James Hyde-Dunn
  • Gareth E. Jones
Part of the Methods in Molecular Biology™ book series (MIMB, volume 75)


The proliferating cell nuclear antigen (PCNA) is the 37-kDa molecular weight auxiliary protein of DNA polymerase δ (1). PCNA is a very conserved and highly regulated protein necessary for cell cycle progression. PCNA is associated with cyclin A in both soluble and insoluble fractions, whereas it is associated with cyclin D1 only in the soluble fraction (2). PCNA translocates from the cytoplasm to the nucleus at the beginning of S-phase, is required for entry into S-phase, and forms multiple high molecular weight complexes with cdk2 at late G1 or early S-phase (2). This cell-cycle distribution of PCNA may be employed as a marker of DNA replication.


Proliferate Cell Nuclear Antigen Gentle Agitation Cell Side Bovine Serum Albumin Concentration Nonspecific Background Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Bravo, R., Frank, R., Blundell, P. A., and MacDonald-Bravo, H. (1987) Cyclin/PCNA is the auxiliary protein of DNA polymerase δ. Nature (Lond.) 326, 515–517.CrossRefGoogle Scholar
  2. 2.
    Prosperi, E., Scovassi, A. I., Stivala, A. L., and Bianchi, L. (1994) Proliferating cell nuclear antigen bound to DNA synthesis sites phosphorylation and association with cyclin D1 and cyclin A. Exp. Cell Res. 215, 257–262.PubMedCrossRefGoogle Scholar
  3. 3.
    Woods, A. L., Hall, P. A., Shepherd, N. A., Hanby A. M., Waseem, N. H., Lane, D. P., and Levison, D. A. (1991) The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis. Histopathology 19, 21–27.PubMedCrossRefGoogle Scholar
  4. 4.
    Waseem, N. H. and Lane, D. P. (1990) Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). J. Cell Sci. 96, 121–129.PubMedGoogle Scholar
  5. 5.
    Beppu, T. and Ishida, Y (1994) Detection of S phase cells with an antibody to proliferating cell nuclear antigen (PCNA). J. Histotechnol. 17(4), 325–328.CrossRefGoogle Scholar
  6. 6.
    Yablonka-Reuveni, Z. and Rivera, A. J. (1994) Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibres. Devel. Biol. 164, 588–603.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1997

Authors and Affiliations

  • James Hyde-Dunn
    • 1
  • Gareth E. Jones
    • 1
  1. 1.Department of Anatomy, The Randall InstituteKing’s College LondonUK

Personalised recommendations