Visualization of Cell Replication Using Antibody to Proliferating Cell Nuclear Antigen
The proliferating cell nuclear antigen (PCNA) is the 37-kDa molecular weight auxiliary protein of DNA polymerase δ (1). PCNA is a very conserved and highly regulated protein necessary for cell cycle progression. PCNA is associated with cyclin A in both soluble and insoluble fractions, whereas it is associated with cyclin D1 only in the soluble fraction (2). PCNA translocates from the cytoplasm to the nucleus at the beginning of S-phase, is required for entry into S-phase, and forms multiple high molecular weight complexes with cdk2 at late G1 or early S-phase (2). This cell-cycle distribution of PCNA may be employed as a marker of DNA replication.
KeywordsProliferate Cell Nuclear Antigen Gentle Agitation Cell Side Bovine Serum Albumin Concentration Nonspecific Background Staining
- 3.Woods, A. L., Hall, P. A., Shepherd, N. A., Hanby A. M., Waseem, N. H., Lane, D. P., and Levison, D. A. (1991) The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis. Histopathology 19, 21–27.PubMedCrossRefGoogle Scholar