Human Long-Term Bone Marrow Culture
The bone marrow is the primary site of hematopoiesis in adults. Accurate in vitro models of hematopoietic regulation and function should reflect the various hormonal and environmental regulators that act on developing hematopoietic cells in the marrow. Some of the regulatory elements acting in vivo are maintained when bone marrow cells are cultured in vitro under suitable culture conditions. Modifications of techniques initially described by Dexter (1; see Chapter 16) for culture of murine bone marrow led to the establishment of an in vitro system that supports human hematopoietic cells (2,3). Functional hematopoiesis in these cultures was dependent on the formation, over 3–4 wk, of an adherent layer of cells derived from structural elements of bone marrow. This layer, termed “stromal” layer because of its likely derivation from the supportive elements in the marrow, has been extensively characterised (4). Stromal cells have been shown to exert positive and negative regulatory effects on hematopoietic cells through cell-cell interaction and by the secretion of cytokines (5,6).
KeywordsMurine Bone Marrow Human Hematopoietic Cell Negative Regulatory Effect Hematopoietic Activity Hematopoietic Regulation
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