Abstract
Steroid hormone receptors activate and repress gene transcription through an integrated series of protein-protein interactions. One principle contact is the interaction of the nuclear receptors with one another to form both homodimeric and heterodimeric complexes that bind with high affinity to their cognate DNA-responsive elements. Following receptor-response element binding, the DNA-bound homodimers and heterodimers must ultimately communicate with the preinitiation complex to influence RNA polymerase II-mediated transcrrption. This communication process presumably involves additional proteinprotein contacis. Identifying these contacis, the proteins that are involved, and the precise nature of these interactions are central to our understanding of this fundamental mechanism.
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References
Fields, S., and Sternglanz, R. (1994) The two-hybrid system· an assay for proteinprotein interactions. Trends Genet 10, 286–292
MacDonald, P N., Sherman, D. R., Dowd, D. R, Jefcoat, S. C., and DeLisle, R. K (1995) The vitamin D receptor interacis with general transcription factor IIB. J. Biol. Chem. 270, 4148–4752.
Ing, N. H., Beekman, J M., Tsai, S. Y., Tsai, M.-J, and O′Malley, B W. (1992) Members of the steroid hormone receptor superfamily interact with TFIIB (S300-II). J. Biol. Chem. 267, 17,617–17,623.
Baniahmad, A., Ha, I., Reinberg, D., Tsai, S., Tsai, M-J., and O′Malley, B W (1993) Interaction of human thyroid hormone receptor ß with transcription factor TFIIB may mediate target gene derepression and activation by thyroid hormone. Proc. Natl. Acad. Sci USA 90, 8832–8836.
Blanco, J. C G., Wang, I-M., Tsai, S. Y, Tsai, M.-J, O’Malley, B W., Jurutka, P W., Haussler, M. R., and Ozato, K. (1995) Transcription factor TFIIB and the vitamin D receptor cooperatively activate ligand-dependent transcription. Proc Natl. Acad Sci. USA 92, 1535–1539.
Onate, S. A, Tsai, S Y., Tsai, M.-J, and O′Malley, B. W (1995) Sequence and characterization of a coactivator for the steroid hormone receptor superfamily Science 270, 1354–1357
Horlein, A. J., Naar, A. M, Hemzel, T., Torchia, J., Gloss, B., Kurokawa, R, Ryan, A., Kamer, Y., Soderstrom, M, Glass, C. K, and Rosenfeld, M. G (1995) Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor. Nature 377, 397–404.
Chen, J D., and Evans, R. M. (1995) A transcriptional corepressor that interacis with nuclear hormone receptors. Nature 377, 454–457
Lee, J W, Ryan, F., Swaffield, J C., Johnston, S A., and Moore, D. D (1995) Interaction of thyroid-hormone receptor wtth a conserved transcriptional mediator Nature 374, 91–94.
Ma, J and Ptashne, M (1987) A new class of yeast transcriptional activators Cell 51, 113–119
Durfee, T, Becherer, K, Chen, P-L, Yeh, S-H., Yang, Y, Kilburn, A E, Lee, W.-H., and Elledge, S. J. (1993) The retinoblastoma protein associates with the protein phosphatase type I catalytic subuint. Genes Dev 7, 555–569
Hannon, G J., Demetrick, D, and Beach, D (1993) Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins Genes Dev. 7, 2378–2391
Yu, V. C., Delsert, C, Andersen, B., Holloway, J M, Devary, O. V, Naar, A M., Kim, S. Y, Boutin, J-M, Glass, C K, and Rosenfeld, M G (1991) RXRß. a coregulator that enhances binding of retinoic acid, thyroid hormone, and vitamin D receptors to their cognate response elements. Cell 67, 1251–1266
Kliewer, S A., Umesono, K., Mangelsdorf, D J, and Evans, R M (1992) Retinoid X receptor interacis with nuclear receptors in retinoic acid, thyroid hormone, and vitamin D3 signalling. Nature 355, 446–449
Nakajima, S, Hsieh, J.-C., MacDonald, P. N, Galligan, M. A, Haussler, C A, Whitfield, G. K, and Haussler, M R (1994) The C-terminal region of the vitamin D receptor is essential to form a complex with a receptor auxiliary factor required for high affinity binding to the vitamin D-responsive element Mol Endocrinol 8, 159–172.
Bartel, P, Chien, C, Sternglanz, R, and Fields, S (1993) Elimination of false positives that arise in using the two-hybrid system. Biotechniques 14, 920–924
Sherman, F. (1991) Getting started with yeast. Meth Enzymol 194, 3–21.
Treco, D. A (1989) Basic techniques of yeast genetics, in Current Protocols in Molecular Biology, Vol. 2 (Ausubel, F M., Brent, R, Kingston, R. E, Moore, D. D, Seidman, J. G, Smith, J A, and Struhl, K, eds.), Wiley, New York, pp.13.1.1–13.2.11
Schiestl, R H and Gietz, R D (1989) High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier Curr. Genet 16, 339–346
Gietz, D., Jean, A. S, Woods, R. A, and Schiestl, R H (1991) An improved method for high efficiency transformation of intact yeast cells Nucl Acids Res 20, 1425
Fagan, R., Flint, K. J, and Jones, N. (1994) Phosphorylation of E2F-1 modulates its interaction with the retinoblastoma gene product and the adenoviral E4 19 kDa protein. Cell 78, 799–811
Reynolds, A., and Lundblad, V. (1989) Yeast vectors and assays for expression of cloned genes, in Current Protocols in Molecular Biology, vol. 2 (Ausubel, F. M, Brent, R, Kingston, R E., Moore, D D, Seidman, J G, Smith, J. A, and Struhl, K., eds.), Wiley, New York, pp 13.6 1–13 6 4
MacDonald, P. N., Dowd, D R., Nakajima, S, Galligan, M A., Reeder, M C, Haussler, C. A., Ozato, K, and Haussler, M. R. (1993) Retinoid X receptors stimulate and 9-cis retinoic acid inhibits 1,25-dihydroxyvitamin D3-activated expression of the rat osteocalcin gene. Mol Cell. Biol. 13, 5907–5917.
Le Douarin, B, Zechel, C., Garnier, J.-M., Lutz, Y, Tora, L., Pierrat, B., Heery, D., Gronemeyer, H., Chambon, P., and Losson, R. (1995) The N-terminal part of TIFl, a putative mediator of the ligand-dependent activation function (AF-2) of nuclear receptors, is fused to B-raf in the oncogenic protein T18. EMBO J. 14, 2020–2033
Robzyk, K., and Kassir, Y. (1992) A simple and highly efficient procedure for rescuing autonomous plasmids from yeast. Nucl. Acids Res. 20, 3790.
Seidman, C. E. (1989) Introduction of plasmid DNA into cells, in Current Protocols in Molecular Biology, vol. 1 (Ausubel, F. M., Brent, R., Kingston, R. E, Moore, D D, Seidman, J. G., Smith, J A, and Struhl, K., eds ), Wiley, New York, pp. 1.8.1–1.8 3
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MacDonald, P.N. (1998). A Two-Hybrid Protein Interaction System to Identify Factors That Interact with Retinoid and Vitamin D Receptors. In: Redfern, C.P.F. (eds) Retinoid Protocols. Methods in Molecular Biology, vol 89. Humana Press. https://doi.org/10.1385/0-89603-438-0:359
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DOI: https://doi.org/10.1385/0-89603-438-0:359
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