Abstract
Retinoic acid receptors (RARs) have been detected in nuclear extracis from various cell types on the basis of their ability to bind radiolabeled retinoic acid (1–3). Prior to the isolation of RAR cDNAs in 1987 (4, 5), biochemical evidence for the existence of nuclear retinoic-acid binding proteins distinct from cytoplasmic CRABP had been obtained (1). The techniques used in these experiments can be used to study the biochemical properties of the RARs and may also be applicable to the study of the related receptors known as RXRs, which bind 9-cis retinoic acid with high affinity (6).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Daly, A K., and Redfern, C. P F (1987) Characterisation of a retinoic-acid binding component from F9 embryonal carcinoma cell nuclei Eur.J. Biochem 68, 133–139
Darmon, M, Rocher, M, Cavey, M.-T., Martin, B., Rabilloud, T, Delescluse, C, and Shroot, B (1988) Biological actirvity of retinoids correlates with affinity for nuclear receptors but not for cytosolic binding protein Skin Pharmacol 1, 161–175
Nervi, C, Grippo, J. F, Sherman, M I, George, M. D, and Jetten, A M. (1989) Identification and characterization of nuclear retinoic acid-binding activity in human myeloblastic leukemia HL-60 cells. Proc. Natl Acad Sci USA 86, 5854–5858.
Petkovich, M., Brand, N J., Krust, A, and Chambon, P (1987) A human retinoic acid receptor which belongs to the family of nuclear receptors. Nature 330, 624–629
Brand, N. J., Petkovich, M, Krust, A., Chambon, P, De Thé, H., Marchio, A., Tiollais, P., and Dejean, A. (1988) Identification of a second human retinoic acid receptor. Nature 332, 850–853.
Levin, A. A, Sturzenbecker, L. J., Kazmer, S., Bosakowski, T., Huselton, C., Allenby, G., Speck, J., Kratzeisen, C., Rosenberger, M., Lovey, A, and Grtppo, J F (1992) 9-cis retinoic acid stereotsomer binds and activates the nuclear receptor RXR-alpha. Nature 355, 359–361
Cavey, M T., Martin, B, Carlavan, I., and Shroot, B. (1990) In vitro binding of retinoids to the nuclear retinolc acid receptor α Anal Biochem 186, 19–23
McCormick, A. M., Napoli, J L., and DeLuca, H. F (1980) High-pressure liquid chromatography of Vitamin A metabohtes and analogs Methods in Enzymol 67, 220–233.
Daly, A. K, Rees, J R, and Redfern, C P F. (1989) Nuclear retinoic-acidbinding proteins and receptors in retinoic-acid-responsive cell lines. Exp Cell. Biol. 57, 339–345.
Muramatsu, M (1970) Isolation of nuclei and nucleoli, in Methods in Cell Physiology, vol. 4 (Prescott, D M., ed.), Academic, New York, NY, pp. 195–230.
Goodman, D. S (1984) Plasma retinol-binding protein, in The Retinoids, vol. 2 (Sporn, M B, Roberts, A B, and Goodman, D. S, eds.), Academic, New York, NY, pp. 41–88
Lewis, C D, Lebkowski, J S, Daly, A K, and Laemmli, U K (1984) Interphase nuclear matrix and metaphase scaffolding structures J. Cell. Sci Suppl. 1, 103–122.
Littlewood, T. D, Hancock, D. C., and Evan, G I. (1987) Characterrzation of a heat shock-induced insoluble complex in the nuclei of cells J. Cell Sci. 88, 65–72.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc, Totowa, NJ
About this protocol
Cite this protocol
Daly, A.K., Redfern, C.P.F. (1998). Isolation of Retinoid Receptors from Manimalian Cells. In: Redfern, C.P.F. (eds) Retinoid Protocols. Methods in Molecular Biology, vol 89. Humana Press. https://doi.org/10.1385/0-89603-438-0:269
Download citation
DOI: https://doi.org/10.1385/0-89603-438-0:269
Publisher Name: Humana Press
Print ISBN: 978-0-89603-438-9
Online ISBN: 978-1-59259-573-0
eBook Packages: Springer Protocols