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Enumeration of Hydrocarbon-Degrading Bacteria

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Bioremediation Protocols

Part of the book series: Methods in Biotechnologyâ„¢ ((MIBT,volume 2))

Abstract

Petroleum products (e.g., gasoline, diesel fuel, creosote) that pollute soil and water are often removed usmg bioremediation that depends on the activities of hydrocarbon-degrading microorganisms. Typically, nutrients and/or electron acceptors are placed in the site to stimulate the growth and activities of these bacteria. Because such abiotic processes as dilution, sorption, or volatilization can be responsible for hydrocarbon disappearance, criteria other than pollutant disappearance must be used (1,2) to prove that microorganisms are responsible and to assess the effective, killed bie, killed bieness of various treatment protocols. Increases in the numbers of hydrocarbon-degrading bacteria in polluted sites as broremediation progresses provide evidence that they are responsible for pollutant disappearance.

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References

  1. Madsen, E L. (1991) Determining in situ biodegradation Facts and challenges.Environ SCL Technol 25 1663–1669

    Google Scholar 

  2. Madsen, E. L., Smclau, J. L., and Ghlorse, W C (1991) In situ biodegradation microbiologlcal patterns in a contaminated aquifer. Science 252, 830–833

    Article  PubMed  CAS  Google Scholar 

  3. Walker, J D and Colwell, R R. (1976) Enumeration of petroleum-degrading mlcroorganisms Appl Envzron Mcrobzol. 31, 198–207

    CAS  Google Scholar 

  4. Leadbetter, E R. and Foster, J W (1958) Studies on some methane-utilizmg bacterra Arch. Mlkrobzol. 30, 91–118

    CAS  Google Scholar 

  5. Wamwright, M., Barakah, F., Al-Turk, I., and Alt, T. A. (1991) Oligotrophic micro-orgamsms in industry, medicme and the environment. Sci Prog (Edinburgh) 75, 313–322

    Google Scholar 

  6. Randall, J D. and Hemmmgsen, B. B. (1994) Evaluation of mineral agar plates for the enumeration of hydrocarbon-degrading bacteria J. Mlcroblol Methods 20, 103–113

    Article  Google Scholar 

  7. Mills, A. L., Breml, C., and Colwell, R R (1978) Enumeration of petroleum degrading marine and estuarine micro organisms by the most probable number method. Can J Mlcroblol. 24, 552–557

    Article  Google Scholar 

  8. Roubal, G and Atlas, R M. (1978) Distribution of hydrocarbon-utilizing micro organisms and hydrocarbon biodegradation potentials in Alaskan contmental shelf areas Appl. Envwon. Mlcrobiol. 35, 897–905.

    CAS  Google Scholar 

  9. Lehmicke, L G., Williams, R T., and Crawford, R. L. (1979) 14C-most-probablenumber method for enumeration of active heterotrophic mrcroorgamsms in natural waters. Appl. Envtron. Mlcrobiol 38, 641–649

    Google Scholar 

  10. Korth, K G (1992) Microbial population changes during bioremedration of gasoline contaminated aquifer water use of a gene probe to enumerate toluene-and xylene-degrading bacteria contammg TOL plasmids M S thesis, San Diego State University, San Diego, CA

    Google Scholar 

  11. Assinder, S. J and Williams, P. A. (1990) The TOL plasmids. determinants of the catabolism of toluene and the xylenes. Adv. Microbial Physiol. 13, 1–69

    Article  Google Scholar 

  12. Kiyohara, H., Nagao, K., and Yana, K (1982) Rapid screen for bacteria degrading water-msoluble, sohd hydrocarbons on agar plates Appl. Environ Microbiol 43, 454–457

    PubMed  CAS  Google Scholar 

  13. Shiaris, M P. and Cooney, J. J (1983) Replica plating method for estimating phenanthrene-utilizing and phenanthrene-cometabohzmg micro organisms. Appl.Environ. Microbiol. 45, 706–710

    PubMed  CAS  Google Scholar 

  14. Bogardt, A H and Hemmmgsen, B B. (1992) Enumeration of phenanthrenedegrading bacteria by an overlayer technique and its use in evaluation of petroleum contaminated sites Appl. Environ. Mlcrobiol. 58, 2579–2582.

    CAS  Google Scholar 

  15. Guerm, W F (1989) Phenanthrene degradation by estuarme surface microlayer and bulk water mrcrobial populations. Mzcrob Ecol 17, 89–104.

    Article  Google Scholar 

  16. Brown, E J and Braddock, J F (1990) Sheen screen, a munaturrzed most-probable-number method for enumeration of oil degrading microorganisms. Appl.Environ Mlcrobiol 563895–3896

    CAS  Google Scholar 

  17. Hames, J. R., Wrenn, B A., Holder, E L., Strohmeier, K. L., Herrmgton, R. T.,and Venosa, A D (1996) Measurement of hydrocarbon-degradmg microbial populations by a 96-well plate most-probable-number procedure. J. Indust. Microbiol 16, 36–41

    Article  Google Scholar 

  18. Stieber, M., Haeseler, F., Werner, P., and Frimmel, F H. (1994) A rapid screening method for micro-organisms degradmg polycyclic aromatic hydrocarbons in microplates Appl. Microbiol Biotech 40, 753–755.

    Article  CAS  Google Scholar 

  19. Petersen, S O. and Klug, M J (1994) Effects of sievmg, storage, and incubation temperature on the phosphohpid fatty acid profile of a soil microbial community Appl Environ. Microbiol 60, 2421–2430.

    PubMed  CAS  Google Scholar 

  20. Ferguson, R L., Buckley, E. N., and Palumbo, A. V. (1984) Response of marme bacterioplankton to differential filtration and confinement. Appl. Environ. Microbial. 47, 49–55

    CAS  Google Scholar 

  21. Tchan, Y T. and New, P B. (1984) Genus I Azotobacter BeiJermek 1901, 567AL,in Bergey’s Manual of Systematzc BactenoZogy, vol. 1 (Krieg, N. R. and Holt, J.G., eds.), Williams & Wilkins, Baltimore, pp 220–229.

    Google Scholar 

  22. Baumann, P and Baumann, L (1980) The marme Gram-negative Eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcabgenes, in The Prokaryotes (Starr, M P., Truper, H. G., Balows, A., and Schlegel, H. G., eds.), Springer-Verlag, Berlin, pp. 1302–1331

    Google Scholar 

  23. Kepner, R. L., Jr and Pratt, J R (1994) Use of fluorochromes for direct enumeration of total bacteria in environmental samples, past and present Mlcrobiol Rev 58, 603–615

    CAS  Google Scholar 

  24. Clesceri, L. S., Greenberg, A E., and Trussell, R. R., eds (1989) Standard Methods for the Exammanon of Water and Wastewater, 17th ed American Public Health Association, Washington, DC

    Google Scholar 

  25. Klee, A. J. (1993) A computer program for the determmation of most probable number and its confidence limits. J. Mzcrobzol. Methods 18, 91–98.

    Article  Google Scholar 

  26. Rice, L. E. (1993) Microbial degradation of hydrocarbons in marine environments changes in bacterial populations and contaminant levels during bioreactor treatment.M S Thesis, San Diego State University, San Diego, CA

    Google Scholar 

  27. Song, H.-G. and Bartha, R. (1990) Effects of Jet fuel spills on the microbial community of soil. Appl. Environ Microbiol. 56, 646–651

    PubMed  CAS  Google Scholar 

  28. Hobbie, J. E., Daley, R. J., and Jasper, S. (1977) Use of nucleopore filters for counting bacteria by fluorescence microscopy. Appl Environ. Microbiol. 33, 1225–1228.

    PubMed  CAS  Google Scholar 

  29. Gersberg, R M., Korth, K. G., Rice, L. E., Randall, J D., Bogardt, A H., Dawsey, W J., and Hemmmgsen, B. B (1995) Chemical and microbial evaluation of zn-sztu bioremediation of hydrocarbons in anoxic groundwater enriched with nutrients and nitrate. World J Microbial. Blotech 11, 549–558.

    Article  CAS  Google Scholar 

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© 1997 Humana Press Inc, Totowa, NJ

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Rice, L.E., Hemmingsen, B.B. (1997). Enumeration of Hydrocarbon-Degrading Bacteria. In: Bioremediation Protocols. Methods in Biotechnologyâ„¢, vol 2. Humana Press. https://doi.org/10.1385/0-89603-437-2:99

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  • DOI: https://doi.org/10.1385/0-89603-437-2:99

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-437-2

  • Online ISBN: 978-1-59259-482-5

  • eBook Packages: Springer Protocols

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